Key Laboratory of Zoonosis Research, Ministry of Education, Department of Animal Science, College of Animal Sciences, Jilin University, Changchun 130062, China.
Toxins (Basel). 2021 Mar 12;13(3):208. doi: 10.3390/toxins13030208.
Lipopolysaccharide (LPS) is an endotoxin, which may cause immune response and inflammation of bovine mammary glands. Mastitis impairs animal health and results in economic loss. Curcumin (CUR) is a naturally occurring diketone compound, which has attracted widespread attention as a potential anti-inflammatory antioxidant. The purpose of this study is to investigate whether CUR can reduce the damage of bovine mammary epithelial cells (MAC-T) induced by LPS and its underlying molecular mechanism. The MAC-T cell line was treated with different concentrations of LPS and CUR for 24 h. The results showed that CUR rescued the decrease of MAC-T cell viability and cell damage induced by LPS. At the same time, 10 µM CUR and 100 µg/mL LPS were used to treat the cells in the follow-up study. The results showed CUR treatment reduced the accumulation of reactive oxygen species (ROS), the expression of inflammatory cytokines (tumor necrosis factor-a (TNF-α), interleukin-8 (IL-8), IL-6 and IL-1β) and the rate of apoptosis induced by LPS. These effects were associated with the activation of the nuclear factor E2-related factor 2 (NFE2L2)-antioxidant response element (ARE) pathway coupled with inactivation of the nuclear factor-κB (NF-κB) inflammatory and caspase/Bcl2 apoptotic pathways.
脂多糖（LPS）是一种内毒素，可能引起牛乳腺的免疫反应和炎症。乳腺炎会损害动物健康并导致经济损失。姜黄素（CUR）是一种天然存在的二酮化合物，作为一种潜在的抗炎抗氧化剂，已引起广泛关注。本研究旨在探讨 CUR 是否可以减轻 LPS 诱导的奶牛乳腺上皮细胞（MAC-T）损伤及其潜在的分子机制。MAC-T 细胞系用不同浓度的 LPS 和 CUR 处理 24 小时。结果表明，CUR 挽救了 LPS 诱导的 MAC-T 细胞活力和细胞损伤的下降。同时，在后续研究中使用 10 μM CUR 和 100 μg/mL LPS 处理细胞。结果表明，CUR 处理减少了 LPS 诱导的活性氧（ROS）积累、炎症细胞因子（肿瘤坏死因子-α（TNF-α）、白细胞介素-8（IL-8）、IL-6 和 IL-1β）的表达和细胞凋亡率。这些作用与核因子 E2 相关因子 2（NFE2L2）-抗氧化反应元件（ARE）通路的激活有关，同时伴有核因子-κB（NF-κB）炎症和 caspase/Bcl2 凋亡通路的失活。
