Key Laboratory of Zoonosis Research, Department of Animal Science, College of Animal Sciences, Jilin University, Ministry of Education, Changchun 130062, China.
Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China.
Toxins (Basel). 2021 Feb 5;13(2):121. doi: 10.3390/toxins13020121.
Zearalenone (ZEA) is a mycotoxin of the genus that can cause endoplasmic reticulum (ER) stress and Apoptosis in bovine mammary epithelial cells (MAC-T). Polydatin (PD), a glycoside purified from has antioxidant properties. This study aimed to explore whether PD can alleviate ZEA-induced damage on bovine mammary epithelial cells (MAC-T). We found that incasing the concentration of ZEA (0, 7.5, 15, 30, 60, 90, 120, and 240 μM) gradually decreased the cell viability. PD treatment alone at 5, 10, and 20 μM did not affect cell viability. Follow-up studies then applied 30 μM of ZEA and 5 μM of PD to treat cells; the results showed that the ZEA + PD treatment group effectively reduced cell oxidative damage compared with the ZEA treatment group. The qPCR analysis showed that ZEA treatment significantly up-regulated the expression of ER stress-related genes, relative to the control. However, adding PD significantly down-regulated the expression of ER stress-related genes. The cell apoptosis detection results showed that, compared with the ZEA treatment group, the ZEA + PD treatment group down-regulated the gene and up-regulated the gene expressions, which reduced the cell apoptosis rate and Caspase-3 activity. Taken together, these results indicate that PD reduces ZEA-induced apoptosis by inhibiting oxidative damage and ER stress.
玉米赤霉烯酮（ZEA）是镰刀菌属的一种真菌毒素，可导致牛乳腺上皮细胞（MAC-T）内质网（ER）应激和细胞凋亡。虎杖苷（PD）是从虎杖中分离得到的一种糖苷，具有抗氧化作用。本研究旨在探讨 PD 是否能缓解 ZEA 对牛乳腺上皮细胞（MAC-T）的损伤。我们发现，随着 ZEA 浓度（0、7.5、15、30、60、90、120 和 240 μM）的逐渐升高，细胞活力逐渐降低。PD 单独处理 5、10 和 20 μM 时并不影响细胞活力。随后的研究应用 30 μM 的 ZEA 和 5 μM 的 PD 处理细胞，结果表明 ZEA+PD 处理组与 ZEA 处理组相比，能有效降低细胞氧化损伤。qPCR 分析表明，与对照组相比，ZEA 处理组 ER 应激相关基因的表达显著上调。然而，添加 PD 可显著下调 ER 应激相关基因的表达。细胞凋亡检测结果表明，与 ZEA 处理组相比，ZEA+PD 处理组下调了 基因的表达，上调了 基因的表达，降低了细胞凋亡率和 Caspase-3 活性。综上所述，这些结果表明 PD 通过抑制氧化损伤和 ER 应激来减少 ZEA 诱导的细胞凋亡。
