Rangel Marina E O, Oliveira Luana P R, Cabral Aline D, Gois Katharyna C, Lima Marcos V M, Reis Beatriz C A A, Fonseca Fernando L A, Sperança Marcia A, Gehrke Flavia S, Laporta Gabriel Z
Setor de Pós-Graduação, Pesquisa e Inovação, Centro Universitário Saúde ABC (FMABC), Fundação ABC, Santo André 09060-870, Brazil.
Centro de Ciências Naturais e Humanas, Universidade Federal do ABC (UFABC), São Bernardo do Campo 09606-045, Brazil.
Insects. 2021 Mar 16;12(3):248. doi: 10.3390/insects12030248.
In 2018-2019, we conducted mosquito collections in a municipal vehicle impound yard, which is 10 km from the Serra do Mar Environmental Protection Area in Santo André, SP, Brazil. Our aim is to study arboviruses in the impound yard, to understand the transmission of arboviruses in an urban environment in Brazil. We captured the mosquitoes using human-landing catches and processed them for arbovirus detection by conventional and quantitative RT-PCR assays. We captured two mosquito species, (73 total specimens; 18 females and 55 males) and (34 specimens; 27 females and 7 males). The minimum infection rate for DENV-2 was 11.5 per 1000 (CI95%: 1-33.9). The detection of DENV-2 RNA in an female suggests that this virus might occur in high infection rates in the sampled mosquito population and is endemic in the urban areas of Santo André. In addition, Guadeloupe mosquito virus RNA was detected in an female. To our knowledge, this was the first detection of the Guadeloupe mosquito virus in Brazil.
2018年至2019年期间,我们在一个市政车辆扣押场进行了蚊虫采集,该扣押场距离巴西圣保罗州圣安德烈市的滨海山脉环境保护区10公里。我们的目的是研究扣押场中的虫媒病毒,以了解巴西城市环境中虫媒病毒的传播情况。我们使用人饵诱捕法捕获蚊虫,并通过常规和定量逆转录聚合酶链反应(RT-PCR)检测法对其进行虫媒病毒检测。我们捕获了两种蚊虫,(共73个样本;18只雌蚊和55只雄蚊)以及(34个样本;27只雌蚊和7只雄蚊)。登革2型病毒(DENV-2)的最低感染率为每1000只中有11.5只(95%置信区间:1-33.9)。在一只雌蚊中检测到DENV-2 RNA,这表明该病毒在采样的蚊虫种群中可能以高感染率存在,并且在圣安德烈市的城市地区呈地方性流行。此外,在一只雌蚊中检测到了瓜德罗普岛蚊病毒RNA。据我们所知,这是巴西首次检测到瓜德罗普岛蚊病毒。