Mulyatno Kris Cahyo, Kotaki Tomohiro, Yotopranoto Subagyo, Rohmah Etik Ainun, Churotin Siti, Sucipto Teguh Hari, Amarullah Ilham Harlan, Wardhani Puspa, Soegijanto Soegeng, Kameoka Masanori
Indonesia-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Airlangga University.
Institute of Tropical Disease, Airlangga University.
Jpn J Infect Dis. 2018 Jan 23;71(1):58-61. doi: 10.7883/yoken.JJID.2017.117. Epub 2017 Oct 31.
Aedes aegypti and Aedes albopictus are the primary and secondary vectors, respectively, of dengue, the most important arboviral disease in the world. The aim of this study was to detect and serotype dengue viruses (DENV) in the vectors Ae. aegypti and Ae. albopictus in Surabaya, Indonesia. Between 2008 and 2015, 16,605 Aedes mosquitoes were collected in 15 sub-districts of Surabaya. Ae. aegypti was dominant (90.9%), whereas few Ae. albopictus were collected (9.1%). A total of 330 pools of adult Aedes mosquitoes were subjected to the serotyping of DENV by RT-PCR. DENV-1 (52.3%) was the most frequently detected serotype, followed by DENV-2 (40.3%), DENV-4 (4.6%), and DENV-3 (2.8%). The average minimum infection rate for Ae. aegypti in various sub-districts of Surabaya was 7.2 per 1,000 mosquitoes, while that for Ae. albopictus was 0.7 per 1,000 mosquitoes. The results showed that the predominantly circulating DENV serotype in mosquitoes continuously shifted from DENV-2 (2008) to DENV-1 (2009-2012), to DENV-2 again (2013-2014), and then back to DENV-1 (2015). The circulating DENV serotypes in mosquitoes were generally consistent with those in humans. Therefore, the surveillance of infected mosquitoes with DENV might provide an early warning sign for the risk of future dengue outbreaks.
埃及伊蚊和白纹伊蚊分别是登革热的主要和次要传播媒介，登革热是世界上最重要的虫媒病毒病。本研究的目的是检测印度尼西亚泗水市埃及伊蚊和白纹伊蚊这两种媒介中的登革热病毒（DENV）并进行血清分型。2008年至2015年期间，在泗水市的15个分区收集了16,605只伊蚊。埃及伊蚊占主导地位（90.9%），而收集到的白纹伊蚊数量较少（9.1%）。总共330组成年伊蚊样本通过逆转录聚合酶链反应（RT-PCR）进行DENV血清分型。DENV-1（52.3%）是最常检测到的血清型，其次是DENV-2（40.3%）、DENV-4（4.6%）和DENV-3（2.8%）。泗水市各分区埃及伊蚊的平均最低感染率为每1000只蚊子7.2只，而白纹伊蚊为每1000只蚊子0.7只。结果表明，蚊子中主要传播的DENV血清型不断变化，从DENV-2（2008年）转变为DENV-1（2009 - 2012年），再变回DENV-2（2013 - 2014年），然后又回到DENV-1（2015年）。蚊子中传播的DENV血清型通常与人类中的一致。因此，监测感染DENV的蚊子可能为未来登革热疫情的风险提供早期预警信号。
