Ghorbanzadeh Sheish Shahnaz, Emadi Rahmatollah, Ahmadian Mehdi, Sadeghzade Sorour, Tavangarian Fariborz
Materials Engineering Group, Pardis College, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Polymers (Basel). 2021 Mar 16;13(6):913. doi: 10.3390/polym13060913.
One of the best methods to prevent wound infection and speed up wound healing is wound dressing based on nanofiber-polymer scaffolds, which have acceptable antimicrobial performance and appropriate skin regeneration capabilities. In this paper, the electrospinning method was applied to synthesize the polyvinylpyrrolidone-acrylic acid hydrogel (PVPA)-eggshell membrane (ESM)-reduced graphene oxide (rGO) nanosheets nanocomposite dressings with different reduced graphene oxide contents (0, 0.5, 1, and 2 wt.%). Thus, smooth nanofibers were fabricated, including a high amount of rGO, which reduced the fiber diameter. Based on the results, rGO played an important role in water impermeability. The results showed that by increasing the rGO concentration from 0.5 to 2 wt%, the contact angle value increased persistently. Results showed that compared to PVPA-ESM, the mechanical strength and strain of PVPA-ESM/1 wt% rGO significantly enhanced 28% and 23%, respectively. Incorporation of 1 wt% rGO enhanced swelling ratio from 875% for PVPA-ESM to 1235% after 420 min, while increasing the rGO to 2 wt% increased the degradation rate of the composites. According to the in vitro cell culture studies, PVPA-ESM wound dressings with 0.5-1 wt% rGO content enhanced PC12 cell viability compared to the wound dressings without rGO nanosheets. Generally, rGO-loaded PVPA-ESM nanofiber wound dressing can be considered as a potential candidate to be used in skin regeneration applications.
预防伤口感染并加速伤口愈合的最佳方法之一是基于纳米纤维-聚合物支架的伤口敷料，这种敷料具有可接受的抗菌性能和适当的皮肤再生能力。在本文中，采用静电纺丝法合成了具有不同氧化石墨烯含量（0、0.5、1和2 wt.%）的聚乙烯吡咯烷酮-丙烯酸水凝胶（PVPA）-蛋壳膜（ESM）-还原氧化石墨烯（rGO）纳米片纳米复合敷料。由此制备出了光滑的纳米纤维，其中包含大量的rGO，这减小了纤维直径。基于结果，rGO在防水性方面发挥了重要作用。结果表明，通过将rGO浓度从0.5 wt.%提高到2 wt.%，接触角值持续增加。结果显示，与PVPA-ESM相比，PVPA-ESM/1 wt.% rGO的机械强度和应变分别显著提高了28%和23%。加入1 wt.%的rGO后，420分钟后的溶胀率从PVPA-ESM的875%提高到了1235%，而将rGO增加到2 wt.%则提高了复合材料的降解速率。根据体外细胞培养研究，与不含rGO纳米片的伤口敷料相比，rGO含量为0.5-1 wt.%的PVPA-ESM伤口敷料提高了PC12细胞的活力。总体而言，负载rGO的PVPA-ESM纳米纤维伤口敷料可被视为皮肤再生应用中的潜在候选材料。
