Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600036, India.
Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai 600036, India; Department of Chemistry, Indian Institute of Technology Madras, Chennai 600036, India.
J Colloid Interface Sci. 2018 May 1;517:251-264. doi: 10.1016/j.jcis.2018.01.110. Epub 2018 Feb 2.
Treatment of chronic non-healing wounds in diabetes is still a major clinical challenge. Here, we have developed reduced graphene oxide (rGO) loaded isabgol nanocomposite scaffolds (Isab + rGO) to treat normal and diabetic wounds. rGO was synthesized by rapid reduction of graphene oxide (GO) under focused solar radiation. Then, rGO was uniformly dispersed into isabgol solution to prepare Isab + rGO nanocomposite scaffolds. These scaffolds were characterized using various physiochemical techniques. Isab + rGO nanocomposite scaffolds showed suitable cell viability, proliferation, and attachment. In vivo experiments were performed using Wistar rats to study the wound healing efficacy of these scaffolds in normal and diabetic rats. Results revealed that rGO stimulated collagen synthesis, collagen crosslinking, wound contraction, and reduced the wound re-epithelialization time significantly compared to control. Histology and immunohistochemistry analyses showed that Isab + rGO scaffold treatment enhanced angiogenesis, collagen synthesis, and deposition in treated wounds. Isab + rGO scaffold treatment also played a major role in shortening the inflammation phase and recruiting macrophages to enhance the early phase of wound healing. Overall, this investigation showed that Isab + rGO scaffold dressing could significantly accelerate the healing of normal and diabetic wounds.
治疗糖尿病慢性难愈性创面仍然是一个重大的临床挑战。在这里，我们开发了负载还原氧化石墨烯（rGO）的车前子纳米复合材料支架（Isab + rGO）来治疗正常和糖尿病创面。rGO 是通过聚焦太阳辐射下快速还原氧化石墨烯（GO）合成的。然后，rGO 均匀分散到车前子溶液中制备 Isab + rGO 纳米复合材料支架。使用各种物理化学技术对这些支架进行了表征。Isab + rGO 纳米复合材料支架表现出适宜的细胞活力、增殖和附着性。使用 Wistar 大鼠进行体内实验，研究这些支架在正常和糖尿病大鼠中的创面愈合效果。结果表明，与对照组相比，rGO 显著刺激胶原合成、胶原交联、创面收缩，并减少创面再上皮化时间。组织学和免疫组织化学分析表明，Isab + rGO 支架治疗促进了治疗部位的血管生成、胶原合成和沉积。Isab + rGO 支架治疗还在缩短炎症期和募集巨噬细胞以增强创面愈合的早期阶段发挥了重要作用。总的来说，这项研究表明，Isab + rGO 支架敷料可以显著加速正常和糖尿病创面的愈合。
