Chemical Agents Laboratory, Department of Occupational and Environmental Medicine, Epidemiology and Hygiene, Italian Workers' Compensation Authority (INAIL)-, Via Fontana Candida 1, Monte Porzio Catone, 00078 Rome, Italy.
Department of Public Health and Infectious Diseases, University of Rome La Sapienza, P.le Aldo Moro, 5, 00185 Rome, Italy.
Int J Environ Res Public Health. 2021 Mar 16;18(6):3068. doi: 10.3390/ijerph18063068.
Drugs able to affect the auditory and nervous systems and consumed by workers to treatdifferent pathologies can represent a possible source of risk in the work environment. All the target compounds involved in the presented project show ototoxic and/or narcoleptic side effects and, for these reasons, occupational safety organizations have recognized them as potential causes of work injuries. A multiclass method for the analysis of 15 drugs among the most widespread worldwide (belonging to nine different classes including antihistamines, beta-blockers, antidepressants, Z-drugs and opioids), was developed and validated. This study describes a rapid, sensitive and effective method to analyse these substances in whole blood using tailored pre-cut dried blood spots. Detection was achieved with a triple quadrupole mass spectrometer after an easy and simple 'dilute and shoot' solubilisation followed by an UPLC separation. All the issues linked to the use of the dried blood spots and whole blood, such as haematocrit variability, volumetric evaluation and sample carrier choice were carefully studied and managed during method development. From the validation study results it emerged that this approach can be deemed successful thanks to its few pg µL LOQs, good linear intervals, absolute recoveries of no less than 75%, an almost negligible matrix effect and accuracy and precision in line with the European and American guidelines for validation. All the obtained goals have been specifically pursued in order to encourage method diffusion as a primary prevention intervention, even in small private workplaces.
能够影响听觉和神经系统的药物，并被工人用于治疗各种病理，可能成为工作环境中的一个潜在风险源。本项目涉及的所有目标化合物都具有耳毒性和/或嗜睡副作用，因此，职业安全组织已经将它们视为潜在的工作伤害原因。本文开发并验证了一种多类方法，用于分析全球范围内最广泛使用的 15 种药物（属于包括抗组胺药、β受体阻滞剂、抗抑郁药、Z 类药物和阿片类药物在内的九种不同类别）。本研究描述了一种使用定制预切干血斑快速、灵敏和有效的方法来分析这些物质在全血中的含量。检测是使用三重四极杆质谱仪在简单的“稀释和进样”溶解后进行 UPLC 分离来实现的。在方法开发过程中，仔细研究并处理了与干血斑和全血使用相关的所有问题，如红细胞压积变异性、体积评估和样本载体选择。从验证研究结果来看，由于其 pg µL 级别的低检测限、良好的线性范围、不少于 75%的绝对回收率、几乎可以忽略不计的基质效应以及符合欧洲和美国验证指南的准确度和精密度，该方法可以被认为是成功的。所有获得的目标都是为了鼓励该方法的推广作为一种初级预防干预措施，即使是在小型私人工作场所。
