Sijbesma Jürgen W A, van Waarde Aren, Stegger Lars, Dierckx Rudi A J O, Boersma Hendrikus H, Slart Riemer H J A
Department of Nuclear Medicine and Molecular Imaging, University Medical Center Groningen, University of Groningen, Hanzeplein 1, 9713GZ Groningen, The Netherlands.
Department of Nuclear Medicine, University Hospital, University of Münster, Albert-Schweitzer-Campus 1, 48149 Münster, Germany.
Int J Mol Sci. 2021 Mar 22;22(6):3236. doi: 10.3390/ijms22063236.
High protein (HP) diets have been proposed to reduce body weight in humans. The diets are known to alter energy metabolism, which can affect the quality of [F]FDG PET heart images. In this preclinical study, we therefore explore the impact of a prolonged HP diet on myocardial [F]FDG uptake.
C57BL/6J (Black six (Bl6)) and apolipoprotein E-deficient () mice were fed chow, a HP diet, or a low protein (LP) diet for 12 weeks. At baseline and after treatment, the animals were injected with 33.0 MBq of [F]FDG and a 30 min PET/CT scan was made. Myocardial volume and [F]FDG uptake were quantified using PET and the % of body fat was calculated from CT.
Myocardial [F]FDG uptake was similar for all diets at the follow-up scan but an increase between baseline and follow-up scans was noticed in the LP groups. Myocardial volume was significantly smaller in the C57BL HP group compared to the other Bl6 groups. Body weight increased less in the two HP groups compared to the chow and LP groups. Body fat percentage was significantly higher in the LP groups. This effect was stronger in C57BL mice (28.7%) compared to mice (15.1%).
Myocardial uptake of [F]FDG in mice is not affected by increased protein intake but [F]FDG uptake increases when the amount of protein is lowered. A lower body weight and percentage of body fat were noticed when applying a HP diet.
高蛋白（HP）饮食被认为可减轻人体体重。已知这类饮食会改变能量代谢，这可能影响[F]FDG PET心脏图像的质量。因此，在这项临床前研究中，我们探讨长期高蛋白饮食对心肌[F]FDG摄取的影响。
将C57BL/6J（黑六（Bl6））和载脂蛋白E缺陷型（）小鼠分别喂食普通饲料、高蛋白饮食或低蛋白（LP）饮食12周。在基线期和治疗后，给动物注射33.0 MBq的[F]FDG，并进行30分钟的PET/CT扫描。使用PET对心肌体积和[F]FDG摄取进行定量，并根据CT计算体脂百分比。
在随访扫描中，所有饮食组的心肌[F]FDG摄取相似，但LP组在基线扫描和随访扫描之间出现增加。与其他Bl6组相比，C57BL高蛋白组的心肌体积明显更小。与普通饲料组和LP组相比，两个高蛋白组的体重增加较少。LP组的体脂百分比明显更高。与（15.1%）相比，这种效应在C57BL小鼠中更强（28.7%）。
小鼠心肌对[F]FDG的摄取不受蛋白质摄入量增加的影响，但当蛋白质含量降低时，[F]FDG摄取会增加。采用高蛋白饮食时，体重和体脂百分比降低。
