Chen Hua-Yan, Li Hong-Liang, Pang Hong, Zhu Chao-Dong, Zhang Yan-Zhou
Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
State Key Laboratory of Biocontrol, Ecology and Evolution, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
Insects. 2021 Mar 26;12(4):290. doi: 10.3390/insects12040290.
The cotton mealybug Tinsley (Hemiptera: Pseudococcidae), is an emerging invasive insect pest in China. Hymenopteran parasitoids are the key organisms for suppressing populations of in the field, and therefore could be used as biological agents. Accurate identification of the associated parasitoids is the critical step to assess their potential role in biological control. In this study, we facilitated the identification of the parasitoid composition of using an integrated approach of species delimitation, combining morphology with molecular data. Eighteen Hymenoptera parasitoid species belonging to 11 genera of four families are recognized based on morphological examination and molecular species delimitation of the mitochondrial cytochrome c oxidase 1 () gene and the 28S rDNA using the automatic barcode gap discovery (ABGD) and the Bayesian Poisson tree processes model (bPTP). Among these species, eight species are primary parasitoids with (Girault) (Hymenoptera: Encyrtidae) being the dominant taxon, while the other 10 species are probably hyperparasitoids, with a prevalence of Li & Xu (Hymenoptera: Encyrtidae). These results indicate that parasitoid wasps associated with from China are diverse and the integrated taxonomic approach applied in this study could enhance the accurate identification of these parasitoids that should be assessed in future biological control programs.
扶桑绵粉蚧（半翅目：粉蚧科）是中国一种新出现的入侵害虫。膜翅目寄生蜂是田间抑制扶桑绵粉蚧种群的关键生物，因此可作为生物防治剂。准确鉴定相关寄生蜂是评估其在生物防治中潜在作用的关键步骤。在本研究中，我们采用物种界定的综合方法，将形态学与分子数据相结合，以辅助鉴定扶桑绵粉蚧的寄生蜂组成。基于形态学检查以及使用自动条形码间隙发现法（ABGD）和贝叶斯泊松树过程模型（bPTP）对线粒体细胞色素c氧化酶亚基1（COI）基因和28S核糖体DNA进行分子物种界定，共识别出属于4科11属的18种膜翅目寄生蜂。在这些物种中，有8种是初级寄生蜂，其中以茶细蛾跳小蜂（姬蜂总科：跳小蜂科）为优势类群，而其他10种可能是重寄生蜂，其中以徐氏嗜蚧跳小蜂（姬蜂总科：跳小蜂科）最为常见。这些结果表明，与中国扶桑绵粉蚧相关的寄生蜂种类多样，本研究中应用的综合分类方法能够提高对这些寄生蜂的准确鉴定，而这些寄生蜂应在未来的生物防治计划中进行评估。
