Bioenergy Group, Agharkar Research Institute, G.G Agarkar Road, Pune 411004, Maharashtra, India; Max Planck Institute for Chemical Ecology, Hans-Knöll-Straße 8, Jena 07745, Germany.
Bioenergy Group, Agharkar Research Institute, G.G Agarkar Road, Pune 411004, Maharashtra, India; Savitribai Phule Pune University, Ganeshkhind, Pune 411007, Maharashtra, India.
Mar Genomics. 2021 Oct;59:100864. doi: 10.1016/j.margen.2021.100864. Epub 2021 Mar 31.
Methanosarcina sp. strain MSH10X1, a psychrotrophic methanogen, was isolated from sub-seafloor methane hydrate deposits of Krishna Godavari Basin on India's east coast. The strain could grow from 5 to 40 °C following all three i.e. methylotrophic, acetoclastic, and hydrogenotrophic modes of methanogenesis utilizing different substrates like methanol, trimethylamine, H/CO (80/20), acetate, valerate, isobutyrate, isopropanol, and isobutanol. The genome sequencing and analysis of this strain revealed a circular chromosome of 3,557,383 bp length having 42.47 mol% G + C content, which consisted of 3110 coding genes, 58 tRNA genes, and 3 rRNA operons. The KEGG analysis highlighted the presence of genes responsible for all three modes of methanogenesis. The presence of genes like mtaB, mtaC, and mttB in the genome provided evidence for possible adaptation of strain MSH10X1 in the deep sea's low-temperature conditions.
从印度东海岸克里希纳戈达瓦里盆地海底甲烷水合物沉积物中分离到一株嗜冷产甲烷菌 Methanosarcina sp. 株 MSH10X1。该菌株可以通过三种产甲烷方式（甲基营养型、乙酰营养型和氢营养型）在 5 到 40°C 的温度范围内生长，利用甲醇、三甲胺、H/CO（80/20）、乙酸盐、戊酸盐、异丁酸盐、异丙醇和异丁醇等不同的基质进行生长。对该菌株的基因组测序和分析表明，其染色体为一个 3,557,383bp 的圆形结构，G+C 含量为 42.47 mol%，包含 3110 个编码基因、58 个 tRNA 基因和 3 个 rRNA 操纵子。KEGG 分析突出了与三种产甲烷方式相关的基因的存在。基因组中 mtaB、mtaC 和 mttB 等基因的存在为菌株 MSH10X1 可能在深海低温条件下的适应提供了证据。
