Chamberlain K G, Tong M, Chiu E, Penington D G
University of Melbourne, Department of Medicine, St. Vincent's Hospital, Victoria, Australia.
Thromb Res. 1988 Feb 15;49(4):425-35. doi: 10.1016/0049-3848(88)90245-9.
The megakaryocyte-platelet regeneration time (MPRT) was measured in 16 normal subjects after irreversible monoamine oxidase (MAO) inhibition. When ten subjects were given a single 20 mg dose of Parnate (tranylcypromine) the mean MPRT was 242 +/- 20 hours (linear model) or 212 +/- 22 hours (weighted mean model) with a mean %-linearity of 75 +/- 22%. The renewal of platelet MAO activity was initially retarded for about 2 days, suggesting that inhibition of megakaryocyte MAO had occurred. Six subjects who had taken MAO inhibitors daily for 1-6 months gave longer MPRT values (274 +/- 57 hours (lin.) or 246 +/- 67 hours (w. m.)), but the differences between the groups were not statistically significant. The single-dose MPRT results agree well with those obtained using the irreversible inhibition of cyclo-oxygenase by aspirin, but exceed the platelet life-spans estimated using radiochemical methods by about 24-36 hours. Platelet serotonin levels did not change significantly after a single dose of Parnate, but supine systolic blood pressure rose by 9 +/- 7 mm Hg (p less than 0.01) 24 hours after the dose. Estimation of MPRT by MAO inhibition is a useful alternative to the aspirin method but screening of subjects and clinical supervision is needed to avoid hypertensive complications.
在16名正常受试者中测量了不可逆单胺氧化酶（MAO）抑制后的巨核细胞 - 血小板再生时间（MPRT）。当10名受试者单次服用20 mg的Parnate（反苯环丙胺）时，平均MPRT为242±20小时（线性模型）或212±22小时（加权平均模型），平均线性百分比为75±22％。血小板MAO活性的更新最初延迟约2天，这表明巨核细胞MAO受到了抑制。6名每天服用MAO抑制剂1 - 6个月的受试者的MPRT值更长（274±57小时（线性）或246±67小时（加权平均）），但两组之间的差异无统计学意义。单剂量MPRT结果与使用阿司匹林不可逆抑制环氧化酶获得的结果非常吻合，但比使用放射化学方法估计的血小板寿命长约24 - 36小时。单次服用Parnate后血小板血清素水平无明显变化，但服药后24小时仰卧收缩压升高了9±7 mmHg（p＜0.01）。通过MAO抑制来估计MPRT是阿司匹林方法的一种有用替代方法，但需要对受试者进行筛查和临床监测以避免高血压并发症。
