Wu Jie, Wang Zhi-Tao, Wang Qiong
Department of Hematology and Respiratory, The Eighth People's Hospital of Hefei, Hefei 230011, Anhui Province, China.
Department of Hematology, The 901th Hospital of the Joint Logistics Support Force of PLA, Hefei 230031, Anhui Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2021 Apr;29(2):581-585. doi: 10.19746/j.cnki.issn.1009-2137.2021.02.043.
To investigate the effect of expression level changes of monocytic myeloid-derived suppressor cells (M-MDSC) to related immune function in the patients with primary immune thrombocytopenia (ITP).
Peripheral blood samples were collected from 53 newly diagnosed ITP patients and 30 healthy volunteers. The quantity of M-MDSC, mRNA levels of Arg-1 and iNOS were detected. CD4T, M-MDSC and CD14HLA-DR cells were sorted. CD4T cells were marked by CFSE, and the immunosuppressive mechanism of M-MDSC was analyzed.
The count of M-MDSC in peripheral blood of newly diagnosed ITP patients was significantly higher than that in the control group (P < 0.01). However, the expression level of Arg-1 in peripheral blood was not significantly different between the newly diagnosed ITP group and the control group. But the expression level of iNOS in the newly diagnosed ITP patients was significantly higher than that in the control group (P < 0.01). After treatment, the count of M-MDSC in the patients with ITP was significantly lower than before treatment (P < 0.01), which showed that M-MDSC could significantly inhibit the proliferation and secretion of IFN-γ in CD4T cells.
M-MDSC may be related to the disorder of immune tolerance in the patients with ITP, and may become a new index to monitor the curative efficacy of ITP patients.
探讨原发性免疫性血小板减少症（ITP）患者中单核细胞来源的髓系抑制细胞（M-MDSC）表达水平变化对相关免疫功能的影响。
采集53例新诊断的ITP患者及30例健康志愿者的外周血样本。检测M-MDSC数量、Arg-1和诱导型一氧化氮合酶（iNOS）的mRNA水平。分选CD4T细胞、M-MDSC和CD14 HLA-DR细胞。用羧基荧光素二乙酸琥珀酰亚胺酯（CFSE）标记CD4T细胞，分析M-MDSC的免疫抑制机制。
新诊断的ITP患者外周血中M-MDSC计数显著高于对照组（P<0.01）。然而，新诊断的ITP组与对照组外周血中Arg-1的表达水平无显著差异。但新诊断的ITP患者中iNOS的表达水平显著高于对照组（P<0.01）。治疗后，ITP患者的M-MDSC计数显著低于治疗前（P<0.01），表明M-MDSC可显著抑制CD4T细胞中干扰素-γ（IFN-γ）的增殖和分泌。
M-MDSC可能与ITP患者的免疫耐受紊乱有关，可能成为监测ITP患者疗效的新指标。
