Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow Campus, Lucknow, Uttar Pradesh, 226028, India.
Department of Oral Pathology & Microbiology, King George's Medical University, Lucknow, Uttar Pradesh, 226 003, India.
Eur Arch Otorhinolaryngol. 2021 Oct;278(10):4081-4089. doi: 10.1007/s00405-021-06774-w. Epub 2021 Apr 4.
The risk of oral cancer is strongly related to consumption of tobacco, smoking and drinking alcohol. N-acetyl transferases 1,2 are phase II metabolic enzymes, metabolize aryl and heterocyclic amines which are present in tobacco. NAT2 slows acetylator phenotype and the genotype is related to reduced ability to detoxify these xenobiotic that are carcinogenic to tissues. The aim of our study to determine the risk of oral cancer as well as oral precancerous lesions in North Indian population with polymorphisms in these two N-acetyl transferases 1,2 genes.
A total of 250 patients with pre oral cancer, oral cancer and 250 healthy volunteers were genotypes for the NAT1 and NAT2 gene polymorphisms. Genotypes were identified by PCR and RFLP. Genotype frequencies were evaluated by Chi-square test and risk of disease was estimated by Odds ratio (OR) with 95% confidence interval.
Our results showed that individuals with CT and TT genotypes of NAT1 C > T polymorphism were significantly lower risk of oral diseases (p value = 0.02, OR = 0.60 and p value = 0.04, OR = 0.58, respectively). For NAT2 C > T polymorphism, the TT genotype significantly increased the risk of OSMF (Oral Sub mucous Fibrosis) and Leukoplakia (p value = 0.001, OR = 4.16; p value = 0.002, OR = 4.38, respectively). In contrary, the CC genotype for NAT2 T > C polymorphism increased the risk of OSMF (p value = 0.01, OR = 3.00, 95% CI = 1.31-6.86).
Our study concludes that the NAT1 polymorphism shows protective association with oral diseases and NAT2 polymorphism and haplotypes also influence the susceptibility to oral diseases in North Indian population subjects.
口腔癌的风险与烟草消费、吸烟和饮酒密切相关。N-乙酰基转移酶 1、2 是 II 相代谢酶，代谢存在于烟草中的芳基和杂环胺。NAT2 减缓乙酰化表型，基因型与降低对这些致癌组织的异生物质解毒的能力有关。我们的研究旨在确定这两种 N-乙酰基转移酶 1、2 基因多态性在北印度人群中与口腔癌以及口腔癌前病变相关的风险。
对 250 例口腔癌前病变、口腔癌患者和 250 名健康志愿者进行了 NAT1 和 NAT2 基因多态性的基因分型。通过 PCR 和 RFLP 确定基因型。通过卡方检验评估基因型频率，用比值比（OR）和 95%置信区间估计疾病风险。
我们的结果表明，NAT1 C＞T 多态性的 CT 和 TT 基因型个体患口腔疾病的风险显著降低（p 值=0.02，OR=0.60 和 p 值=0.04，OR=0.58）。对于 NAT2 C＞T 多态性，TT 基因型显著增加 OSMF（口腔黏膜下纤维变性）和白斑（p 值=0.001，OR=4.16；p 值=0.002，OR=4.38）的风险。相反，NAT2 T＞C 多态性的 CC 基因型增加了 OSMF 的风险（p 值=0.01，OR=3.00，95%CI=1.31-6.86）。
我们的研究表明，NAT1 多态性与口腔疾病呈保护相关，NAT2 多态性和单倍型也影响北印度人群口腔疾病的易感性。
