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柏子仁挥发油对储存柏子仁中黄曲霉和黄曲霉毒素的抑菌活性

Antifungal Activity of Essential Oil From DC. on and Aflatoxins in Stored Platycladi Semen.

作者信息

Li Ting, Chen Mingyang, Ren Guangxi, Hua Guodong, Mi Jiu, Jiang Dan, Liu Chunsheng

机构信息

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China.

Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing, China.

出版信息

Front Microbiol. 2021 Mar 19;12:633714. doi: 10.3389/fmicb.2021.633714. eCollection 2021.

Abstract

The major objective of this study was to evaluate the inhibitory effect of essential oil (EO) from DC. on . The chemical composition of the EO was identified by gas chromatography-mass spectrometer. The minimum inhibitory concentration (MIC) of EO was investigated by liquid fermentation. The morphology, colony number, and aflatoxin content of in platycladi semen were investigated by stereomicroscopy, scanning electron microscopy, plate counting, and high-performance liquid chromatography. The results indicated that the MIC of EO was 0.8 μL⋅mL, and the main components were β-phellandrene (7.53%), D-limonene (13.24%), linalool (41.73%), terpinen-4-ol (5.33%), and -nerolidol (6.30%). After the EO fumigated the platycladi semen, the growth of slowed, and the mycelium shrank considerably. The number of colonies after EO treatment at room temperature and cold storage was significantly reduced, the inhibition effect was better under cold storage, and the aflatoxin B1 content did not exceed the standard within 100 days. Therefore, this study demonstrated the good potential of growth inhibition during the storage of platycladi semen.

摘要

本研究的主要目的是评估来自[植物名称未明确写出]的精油(EO)对[具体对象未明确写出]的抑制作用。通过气相色谱 - 质谱联用仪鉴定了该精油的化学成分。采用液体发酵法研究了精油的最低抑菌浓度(MIC)。通过体视显微镜、扫描电子显微镜、平板计数和高效液相色谱法对侧柏叶中的[具体对象未明确写出]的形态、菌落数和黄曲霉毒素含量进行了研究。结果表明,精油的MIC为0.8 μL·mL,主要成分是β - 水芹烯(7.53%)、D - 柠檬烯(13.24%)、芳樟醇(41.73%)、萜品 - 4 - 醇(5.33%)和β - 橙花叔醇(6.30%)。用该精油熏蒸侧柏叶后,[具体对象未明确写出]的生长减缓,菌丝体大幅收缩。在室温和冷藏条件下经精油处理后的菌落数显著减少,冷藏条件下的抑制效果更好,且100天内黄曲霉毒素B1含量未超标。因此,本研究证明了在侧柏叶储存期间[具体对象未明确写出]具有良好的生长抑制潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fcce/8017187/94bd556490a6/fmicb-12-633714-g001.jpg

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