• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

间充质干细胞暴露于阿尔茨海默病环境可增强治疗效果。

Exposure of Mesenchymal Stem Cells to an Alzheimer's Disease Environment Enhances Therapeutic Effects.

作者信息

Park Sang Eon, Kim Hyeong Seop, Kwon Soo Jin, Kim Min-Jeong, Choi Suk-Joo, Oh Soo-Young, Ryu Gyu Ha, Jeon Hong Bae, Na Duk L, Chang Jong Wook

机构信息

Stem Cell Institute, ENCell Co. Ltd, Seoul 06072, Republic of Korea.

Stem Cell & Regenerative Medicine Institute, Samsung Medical Center, Seoul 06351, Republic of Korea.

出版信息

Stem Cells Int. 2021 Mar 16;2021:6660186. doi: 10.1155/2021/6660186. eCollection 2021.

DOI:10.1155/2021/6660186
PMID:33815510
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7988745/
Abstract

Mesenchymal stem cells (MSCs) have emerged as a promising tool for the treatment of Alzheimer's disease (AD). Previous studies suggested that the coculture of human MSCs with AD in an model reduced the expression of amyloid-beta 42 (A42) in the medium as well as the overexpression of amyloid-beta- (A-) degrading enzymes such as neprilysin (NEP). We focused on the role of primed MSCs (human Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) exposed to an AD cell line via a coculture system) in reducing the levels of A and inhibiting cell death. We demonstrated that mouse groups treated with naïve MSCs and primed MSCs showed significant reductions in cell death, ubiquitin conjugate levels, and A levels, but the effects were greater in primed MSCs. Also, mRNA sequencing data analysis indicated that high levels of TGF- induced primed-MSCs. Furthermore, treatment with TGF- reduced A expression in an AD transgenic mouse model. These results highlighted AD environmental preconditioning is a promising strategy to reduce cell death and ubiquitin conjugate levels and maintain the stemness of MSCs. Further, these data suggest that human WJ-MSCs exposed to an AD environment may represent a promising and novel therapy for AD.

摘要

间充质干细胞（MSCs）已成为治疗阿尔茨海默病（AD）的一种有前景的工具。先前的研究表明，在AD模型中，人MSCs与AD共培养可降低培养基中β淀粉样蛋白42（A42）的表达以及诸如中性内肽酶（NEP）等β淀粉样蛋白（Aβ）降解酶的过表达。我们聚焦于预处理的MSCs（通过共培养系统暴露于AD细胞系的人脐带华通氏胶来源的间充质干细胞（WJ-MSCs））在降低Aβ水平和抑制细胞死亡中的作用。我们证明，用未处理的MSCs和预处理的MSCs处理的小鼠组在细胞死亡、泛素缀合物水平和Aβ水平上均有显著降低，但预处理的MSCs效果更明显。此外，mRNA测序数据分析表明，高水平的转化生长因子-β（TGF-β）诱导了预处理的MSCs。此外，在AD转基因小鼠模型中，用TGF-β处理可降低Aβ表达。这些结果突出表明，AD环境预处理是一种有前景的策略，可减少细胞死亡和泛素缀合物水平，并维持MSCs的干性。此外，这些数据表明，暴露于AD环境的人WJ-MSCs可能代表一种有前景的新型AD治疗方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/2e742d011b9a/SCI2021-6660186.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/d6523b46c6e2/SCI2021-6660186.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/f1ba06505b0d/SCI2021-6660186.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/b0297d1828c2/SCI2021-6660186.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/eea9833a4020/SCI2021-6660186.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/0d64f6065039/SCI2021-6660186.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/e79cbf68e540/SCI2021-6660186.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/1e7f2aa9003e/SCI2021-6660186.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/2e742d011b9a/SCI2021-6660186.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/d6523b46c6e2/SCI2021-6660186.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/f1ba06505b0d/SCI2021-6660186.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/b0297d1828c2/SCI2021-6660186.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/eea9833a4020/SCI2021-6660186.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/0d64f6065039/SCI2021-6660186.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/e79cbf68e540/SCI2021-6660186.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/1e7f2aa9003e/SCI2021-6660186.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c318/7988745/2e742d011b9a/SCI2021-6660186.008.jpg

相似文献

1
Exposure of Mesenchymal Stem Cells to an Alzheimer's Disease Environment Enhances Therapeutic Effects.间充质干细胞暴露于阿尔茨海默病环境可增强治疗效果。
Stem Cells Int. 2021 Mar 16;2021:6660186. doi: 10.1155/2021/6660186. eCollection 2021.
2
Wharton's Jelly-derived mesenchymal stem cells alleviate memory deficits and reduce amyloid-β deposition in an APP/PS1 transgenic mouse model.在APP/PS1转基因小鼠模型中，源自华通氏胶的间充质干细胞可减轻记忆缺陷并减少β淀粉样蛋白沉积。
Clin Exp Med. 2016 Feb;16(1):89-98. doi: 10.1007/s10238-015-0375-0. Epub 2015 Jul 19.
3
Priming with Toll-like receptor 3 agonist or interferon-gamma enhances the therapeutic effects of human mesenchymal stem cells in a murine model of atopic dermatitis.TLR3 激动剂或干扰素-γ 预刺激增强人骨髓间充质干细胞在特应性皮炎小鼠模型中的治疗效果。
Stem Cell Res Ther. 2019 Feb 22;10(1):66. doi: 10.1186/s13287-019-1164-6.
4
Extracellular Vesicles Released from Neprilysin Gene-Modified Human Umbilical Cord-Derived Mesenchymal Stem Cell Enhance Therapeutic Effects in an Alzheimer's Disease Animal Model.中性肽链内切酶基因修饰的人脐带间充质干细胞释放的细胞外囊泡增强阿尔茨海默病动物模型的治疗效果。
Stem Cells Int. 2021 Dec 3;2021:5548630. doi: 10.1155/2021/5548630. eCollection 2021.
5
Hypoxia with Wharton's jelly mesenchymal stem cell coculture maintains stemness of umbilical cord blood-derived CD34 cells.缺氧条件下与华通氏胶间充质干细胞共培养维持脐带血来源 CD34 细胞的干性。
Stem Cell Res Ther. 2018 Jun 13;9(1):158. doi: 10.1186/s13287-018-0902-5.
6
Cerebrospinal fluid from Alzheimer's disease patients as an optimal formulation for therapeutic application of mesenchymal stem cells in Alzheimer's disease.阿尔茨海默病患者的脑脊液是间充质干细胞在阿尔茨海默病治疗应用中的最佳配方。
Sci Rep. 2019 Jan 24;9(1):564. doi: 10.1038/s41598-018-37252-9.
7
Priming Wharton's jelly-derived mesenchymal stromal/stem cells with ROCK inhibitor improves recovery in an intracerebral hemorrhage model.用ROCK抑制剂预处理沃顿胶衍生的间充质基质/干细胞可改善脑出血模型中的恢复情况。
J Cell Biochem. 2015 Feb;116(2):310-9. doi: 10.1002/jcb.24969.
8
Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells.人脐带华通氏胶间充质干细胞向子宫内膜细胞的分化。
Stem Cell Res Ther. 2017 Nov 2;8(1):246. doi: 10.1186/s13287-017-0700-5.
9
Effect of growth differentiation factor-15 secreted by human umbilical cord blood-derived mesenchymal stem cells on amyloid beta levels in in vitro and in vivo models of Alzheimer's disease.人脐带血间充质干细胞分泌的生长分化因子-15 对阿尔茨海默病体外和体内模型中β淀粉样蛋白水平的影响。
Biochem Biophys Res Commun. 2018 Oct 12;504(4):933-940. doi: 10.1016/j.bbrc.2018.09.012. Epub 2018 Sep 14.
10
Improving stemness and functional features of mesenchymal stem cells from Wharton's jelly of a human umbilical cord by mimicking the native, low oxygen stem cell niche.通过模拟天然低氧的干细胞龛，提高人脐带华通氏胶间充质干细胞的干性和功能特征。
Placenta. 2019 Jul;82:25-34. doi: 10.1016/j.placenta.2019.05.005. Epub 2019 May 13.

引用本文的文献

1
Molecular Regulation of Fetal Brain Development in Inbred and Congenic Mouse Strains Differing in Longevity.同窝和近交系小鼠在寿命上的差异与胎儿大脑发育的分子调控。
Genes (Basel). 2024 May 9;15(5):604. doi: 10.3390/genes15050604.
2
Mesenchymal Stem Cells Applications in Alzheimer's Disease.间充质干细胞在阿尔茨海默病中的应用
Glob Med Genet. 2023 Dec 11;10(4):382-387. doi: 10.1055/s-0043-1777087. eCollection 2023 Dec.
3
Mesenchymal stromal cell-derived secretome-based therapy for neurodegenerative diseases: overview of clinical trials.

本文引用的文献

1
A Perspective on Multi-target Drugs for Alzheimer's Disease.阿尔茨海默病多靶药物治疗的思考
Trends Pharmacol Sci. 2020 Jul;41(7):434-445. doi: 10.1016/j.tips.2020.04.008. Epub 2020 May 21.
2
Heparan Sulfate Proteoglycans Undergo Differential Expression Alterations in Alzheimer Disease Brains.硫酸乙酰肝素蛋白聚糖在阿尔茨海默病大脑中发生差异表达改变。
J Neuropathol Exp Neurol. 2020 May 1;79(5):474-483. doi: 10.1093/jnen/nlaa016.
3
Genetically modified mesenchymal stem cell therapy for acute respiratory distress syndrome.基因修饰间充质干细胞治疗急性呼吸窘迫综合征。
基于间充质基质细胞衍生的 secretome 的神经退行性疾病治疗：临床试验概述。
Stem Cell Res Ther. 2023 May 4;14(1):122. doi: 10.1186/s13287-023-03264-0.
4
Mesenchymal stem cell- and extracellular vesicle-based therapies for Alzheimer's disease: progress, advantages, and challenges.基于间充质干细胞和细胞外囊泡的阿尔茨海默病治疗方法：进展、优势与挑战
Neural Regen Res. 2023 Aug;18(8):1645-1651. doi: 10.4103/1673-5374.361546.
5
Mesenchymal stromal cells for the treatment of Alzheimer's disease: Strategies and limitations.用于治疗阿尔茨海默病的间充质基质细胞：策略与局限性
Front Mol Neurosci. 2022 Oct 6;15:1011225. doi: 10.3389/fnmol.2022.1011225. eCollection 2022.
6
Combination of Dexamethasone and Tofacitinib Reduces Xenogeneic MSC-Induced Immune Responses in a Mouse Model of Alzheimer's Disease.地塞米松与托法替布联合使用可减轻阿尔茨海默病小鼠模型中异种间充质干细胞诱导的免疫反应。
Biomedicines. 2022 Aug 4;10(8):1882. doi: 10.3390/biomedicines10081882.
7
Mesenchymal Stem Cell Therapy for Alzheimer's Disease.间充质干细胞疗法治疗阿尔茨海默病
Stem Cells Int. 2021 Sep 1;2021:7834421. doi: 10.1155/2021/7834421. eCollection 2021.
Stem Cell Res Ther. 2019 Dec 16;10(1):386. doi: 10.1186/s13287-019-1518-0.
4
PDGFB-expressing mesenchymal stem cells improve human hematopoietic stem cell engraftment in immunodeficient mice.表达血小板衍生生长因子 B 的间充质干细胞可改善免疫缺陷小鼠中的人造血干细胞植入。
Bone Marrow Transplant. 2020 Jun;55(6):1029-1040. doi: 10.1038/s41409-019-0766-z. Epub 2019 Dec 5.
5
Transplantation of Mesenchymal Stem Cells Improves Amyloid-β Pathology by Modifying Microglial Function and Suppressing Oxidative Stress.间质干细胞移植通过调节小胶质细胞功能和抑制氧化应激改善淀粉样β病理。
J Alzheimers Dis. 2019;72(3):867-884. doi: 10.3233/JAD-190817.
6
Neurodegeneration and Neuro-Regeneration-Alzheimer's Disease and Stem Cell Therapy.神经退行性变与神经再生——阿尔茨海默病与干细胞治疗。
Int J Mol Sci. 2019 Aug 31;20(17):4272. doi: 10.3390/ijms20174272.
7
Genetic Stability of Mesenchymal Stromal Cells for Regenerative Medicine Applications: A Fundamental Biosafety Aspect.用于再生医学应用的间充质基质细胞的遗传稳定性：一个基本的生物安全性方面。
Int J Mol Sci. 2019 May 15;20(10):2406. doi: 10.3390/ijms20102406.
8
Priming approaches to improve the efficacy of mesenchymal stromal cell-based therapies.改善间充质基质细胞疗法疗效的启动方法。
Stem Cell Res Ther. 2019 May 2;10(1):131. doi: 10.1186/s13287-019-1224-y.
9
Cerebrospinal fluid from Alzheimer's disease patients as an optimal formulation for therapeutic application of mesenchymal stem cells in Alzheimer's disease.阿尔茨海默病患者的脑脊液是间充质干细胞在阿尔茨海默病治疗应用中的最佳配方。
Sci Rep. 2019 Jan 24;9(1):564. doi: 10.1038/s41598-018-37252-9.
10
Mesenchymal Stromal Cell Secretome: Influencing Therapeutic Potential by Cellular Pre-conditioning.间质基质细胞分泌组：通过细胞预处理影响治疗潜力。
Front Immunol. 2018 Dec 4;9:2837. doi: 10.3389/fimmu.2018.02837. eCollection 2018.