Deenin Wanwisa, Malakul Wachirawadee, Boonsong Tantip, Phoungpetchara Ittipon, Tunsophon Sakara
Department of Physiology, Faculty of Medical Science, Naresuan University, Muang 65000, Phitsanulok, Thailand.
Department of Biochemistry, Faculty of Medical Science, Naresuan University, Muang 65000, Phitsanulok, Thailand.
World J Hepatol. 2021 Mar 27;13(3):315-327. doi: 10.4254/wjh.v13.i3.315.
Non-alcoholic fatty liver disease (NAFLD) is a global health issue that is correlated with obesity and oxidative stress.
To evaluate the anti-NAFLD effect of papaya in high fat diet induced obesity in rats.
Four-week-old male Sprague-Dawley rats were divided into four groups after 1 wk of acclimatization: Group 1 was the rats fed a normal diet (C); group 2 was the rats fed a high fat diet (HFD); group 3 was the rats fed a HFD with 0.5 mL of papaya juice/100 g body weight (HFL), and group 4 was the rats fed a HFD with 1 mL of papaya juice/100 g body weight (HFH) for 12 wk. At the end of the treatment, blood and tissue samples were collected for biochemical analyses and histological assessment.
The results of the HFH group showed significantly reduced body weight (HFH HFD, < 0.01), decreased NAFLD score (HFH HFD, < 0.05), and reduced hepatic total cholesterol (HFL HFD, < 0.01; HFH HFD, < 0.001), hepatic triglyceride (HFH HFD, < 0.05), malondialdehyde (HFL, HFH HFD, < 0.001), tumour necrosis factor-α (HFH HFD, < 0.05) and interleukin-6 (HFH HFD, < 0.05) when compared to the HFD group. However, the liver weight showed no significant difference among the groups. The activities of catalase and superoxide dismutase significantly increased in HFH when compared with the HFD group ( < 0.05 and < 0.001, respectively). The suppression of transcriptional factors of hepatic lipogenesis, including sterol regulatory element-binding protein 1c and fatty acid synthase, were observed in the papaya treated group (HFH HFD, < 0.05). These beneficial effects of papaya against HFD-induced NAFLD are through lowering hepatic lipid accumulation, suppressing the lipogenic pathway, improving the balance of antioxidant status, and lowering systemic inflammation.
These current results provide experimental-based evidence suggesting papaya is an efficacious medicinal fruit for use in the prevention or treatment of NAFLD.
非酒精性脂肪性肝病（NAFLD）是一个全球性的健康问题，与肥胖和氧化应激相关。
评估木瓜对高脂饮食诱导的大鼠肥胖的抗NAFLD作用。
4周龄雄性Sprague-Dawley大鼠在适应1周后分为四组：第1组为喂食正常饮食的大鼠（C）；第2组为喂食高脂饮食的大鼠（HFD）；第3组为喂食高脂饮食且每100克体重给予0.5毫升木瓜汁的大鼠（HFL），第4组为喂食高脂饮食且每100克体重给予1毫升木瓜汁的大鼠（HFH），持续12周。治疗结束时，采集血液和组织样本进行生化分析和组织学评估。
HFH组结果显示体重显著降低（HFH＜HFD，P＜0.01），NAFLD评分降低（HFH＜HFD，P＜0.05），肝总胆固醇降低（HFL＜HFD，P＜0.01；HFH＜HFD，P＜0.001），肝甘油三酯降低（HFH＜HFD，P＜0.05），丙二醛降低（HFL、HFH＜HFD，P＜0.001），肿瘤坏死因子-α降低（HFH＜HFD，P＜0.05）以及白细胞介素-6降低（HFH＜HFD，P＜0.05），与HFD组相比。然而，各组间肝脏重量无显著差异。与HFD组相比，HFH组过氧化氢酶和超氧化物歧化酶活性显著增加（分别为P＜0.05和P＜0.001）。在木瓜治疗组中观察到肝脏脂肪生成转录因子的抑制，包括固醇调节元件结合蛋白1c和脂肪酸合酶（HFH＜HFD，P＜0.05）。木瓜对高脂饮食诱导的NAFLD的这些有益作用是通过降低肝脏脂质积累、抑制脂肪生成途径、改善抗氧化状态平衡以及降低全身炎症来实现的。
这些当前结果提供了基于实验的证据，表明木瓜是用于预防或治疗NAFLD的有效药用水果。
