Nicklas W, Mauter P
Zentrales Tierlabor, Deutsches Krebsforschungszentrum, Heidelberg.
Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Mar;267(4):510-8.
Over an eight years period about 6200 cell cultures, sera, cell culture media and supernatants were routinely monitored for contamination with mycoplasmas, bacteria and fungi. Mycoplasmas were detected in 24.0% of 4443 samples which were checked for possible contamination. In 1742 samples from a laboratory, known to have only mycoplasma free cultures, 2 were positive, both samples having an external origin. The value of routine monitoring to prevent the introduction of mycoplasma was confirmed. Culture and direct fluorescent assay using the fluorochrome bisbenzimide (Hoechst 33258) yielded comparable results. The applicability and significance of both methods is discussed. In spite of a few disadvantages the culture method is considered to be superior to the fluorescence assay, but both methods should be employed in order to obtain sufficiently reliable results. The importance of appropriate methods for the detection of mycoplasmas is stressed because of their potential influence on experimental results. The probable sources of cell culture contamination are also discussed.
在八年的时间里，对约6200份细胞培养物、血清、细胞培养基和上清液进行了支原体、细菌和真菌污染的常规监测。在4443份检查是否可能被污染的样本中，24.0%检测到支原体。在一个已知只有无支原体培养物的实验室的1742份样本中，有2份呈阳性，这两份样本均来自外部。常规监测对防止支原体引入的价值得到了证实。使用荧光染料双苯甲酰亚胺（Hoechst 33258）进行培养和直接荧光检测得到了可比的结果。讨论了这两种方法的适用性和意义。尽管培养法有一些缺点，但仍被认为优于荧光检测法，但两种方法都应采用，以便获得足够可靠的结果。强调了适当的支原体检测方法的重要性，因为它们可能对实验结果产生影响。还讨论了细胞培养污染的可能来源。
