Fatima Syeda, Aslam Nazia, Khalid Sofia, Ullah Kalim, Abbas Khizar, Hussain Shahzad, Shah Syed Sajid Hussain, Qureshi Zia-Ur-Rahman, Qayum Mughal, Asad Muhammad Hassham Hassan Bin
Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, Pakistan.
Department of Zoology, Kohat University of Science and Technology (KUST), Kohat, Pakistan.
Biomed Res Int. 2021 Mar 16;2021:6618349. doi: 10.1155/2021/6618349. eCollection 2021.
Snakebite is one of the most neglected diseases of developing countries. Deaths due to snakebite envenoming are quite high in Pakistan, and many deaths are caused by envenomation. Traditional use of medicinal plants against snakebites is a common practice in Pakistan due to countless benefits. The current study was performed with the objective to evaluate eighteen Pakistani medicinal plants inhibitory potential against hyaluronidase and alkaline phosphatase enzymes of Pakistani venom. Hyaluronidase activity (0.2-1.6 mg/0.1 mL) and alkaline phosphatase activity (0.1-0.8 mg/0.1 mL) were measured in dose-dependent manner. Crude methanolic extracts of medicinal plants were used for in vitro investigation of their inhibitory activity against toxic enzymes. All active plants were fractioned using different solvents and were again analyzed for inhibitory activity of same enzymes. Results indicated all plants were able to neutralize hyaluronidase that (Roxb. ex Flem.) Karst., Wight and Arn, Thumb., and (L.) R.Br. inhibited maximum hyaluronidase activity equivalent to standard reference ( > 0.5). Pakistani medicinal plants are dense with natural neutralizing metabolites and other active phytochemicals which could inhibit hyaluronidase activity of Pakistani venom. Further advanced studies at molecular level could lead us to an alternative for envenoming of Pakistani venom.
蛇咬伤是发展中国家最被忽视的疾病之一。在巴基斯坦，因蛇咬中毒导致的死亡人数相当高，许多死亡是由中毒引起的。由于有诸多益处，在巴基斯坦，传统上使用药用植物治疗蛇咬伤是一种常见做法。本研究旨在评估18种巴基斯坦药用植物对巴基斯坦毒液中的透明质酸酶和碱性磷酸酶的抑制潜力。以剂量依赖方式测量透明质酸酶活性（0.2 - 1.6毫克/0.1毫升）和碱性磷酸酶活性（0.1 - 0.8毫克/0.1毫升）。药用植物的粗甲醇提取物用于体外研究其对有毒酶的抑制活性。所有活性植物用不同溶剂进行分离，并再次分析相同酶的抑制活性。结果表明所有植物都能够中和透明质酸酶，其中[具体植物名称1]、[具体植物名称2]、[具体植物名称3]和[具体植物名称4]抑制的透明质酸酶活性最高，相当于标准参考物（>0.5）。巴基斯坦药用植物富含天然中和代谢物和其他活性植物化学物质，它们可以抑制巴基斯坦毒液的透明质酸酶活性。在分子水平上进一步深入研究可能会为我们找到治疗巴基斯坦毒液中毒的替代方法。
