Janardhan Bhavya, Shrikanth Vineetha M, More Veena S, Melappa Govindappa, Zameer Farhan, More Sunil S
Department of Biochemistry, Center for Post Graduate Studies, Jain University, India.
Department of Biotechnology, Sapthagiri College of Engineering, Bangalore, 57, India.
Heliyon. 2019 Jul 29;5(7):e02163. doi: 10.1016/j.heliyon.2019.e02163. eCollection 2019 Jul.
Lam. is native to Africa and India. The plant and its parts are used for treating various ailments including snake bites. The different concentrations of ethyl acetate leaf extract of were used to neutralize the toxic effect of venom through dose dependent enzyme studies and studies. The extract was able to neutralize the 5' nucleotidase, phospholipase A, Phosphodiesterae, phosphomonoesterase, acetylcholinesterase and hyaluronidase in a dose dependent manner with concentrations ranging from 43.98 -340.1 μg/mL of extract. The extract could retain the lysis of fibrinogen at the concentration of 1:10 (venom: extract, w/w) and also the lysis of lecithin was reduced at a concentration of 1:25 (venom: extract, w/w). The extract was able to neutralize the LD of venom in both mice and embryo and also reduce the myotoxic and edema properties of the venom in mice models. The venom did not show any procoagulant and hemorrhagic effect. The leaf extract possess adequate compounds/phytochemicals that could neutralize the toxic properties/activity of the venom.
Lam.原产于非洲和印度。该植物及其各部分用于治疗包括蛇咬伤在内的各种疾病。通过剂量依赖性酶研究和其他研究，使用不同浓度的该植物乙酸乙酯叶提取物来中和毒液的毒性作用。提取物能够以剂量依赖性方式中和5'核苷酸酶、磷脂酶A、磷酸二酯酶、磷酸单酯酶、乙酰胆碱酯酶和透明质酸酶，提取物浓度范围为43.98 - 340.1μg/mL。提取物在1:10（毒液：提取物，w/w）浓度下可保留纤维蛋白原的溶解，在1:25（毒液：提取物，w/w）浓度下卵磷脂的溶解也会降低。提取物能够中和小鼠和胚胎中毒液的半数致死量，并且还能降低小鼠模型中毒液的肌毒性和水肿特性。毒液未显示出任何促凝血和出血作用。叶提取物含有足够的化合物/植物化学物质，可以中和毒液的毒性特性/活性。
