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绘制SVG图形以可视化并在染色体模式图上绘制全基因组数据。

: drawing SVG graphics to visualize and map genome-wide data on the idiograms.

作者信息

Hao Zhaodong, Lv Dekang, Ge Ying, Shi Jisen, Weijers Dolf, Yu Guangchuang, Chen Jinhui

机构信息

Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, Jiangsu, China.

Laboratory of Biochemistry, Wageningen University, Wageningen, Haarlem, Netherlands.

出版信息

PeerJ Comput Sci. 2020 Jan 20;6:e251. doi: 10.7717/peerj-cs.251. eCollection 2020.

Abstract

BACKGROUND

Owing to the rapid advances in DNA sequencing technologies, whole genome from more and more species are becoming available at increasing pace. For whole-genome analysis, idiograms provide a very popular, intuitive and effective way to map and visualize the genome-wide information, such as GC content, gene and repeat density, DNA methylation distribution, genomic synteny, etc. However, most available software programs and web servers are available only for a few model species, such as human, mouse and fly, or have limited application scenarios. As more and more non-model species are sequenced with chromosome-level assembly being available, tools that can generate idiograms for a broad range of species and be capable of visualizing more data types are needed to help better understanding fundamental genome characteristics.

RESULTS

The R package allows users to build high-quality idiograms of any species of interest. It can map continuous and discrete genome-wide data on the idiograms and visualize them in a heat map and track labels, respectively.

CONCLUSION

The visualization of genome-wide data mapping and comparison allow users to quickly establish a clear impression of the chromosomal distribution pattern, thus making a useful tool for any researchers working with omics.

摘要

背景

由于DNA测序技术的飞速发展,越来越多物种的全基因组正以越来越快的速度被获取。对于全基因组分析而言,染色体模式图提供了一种非常流行、直观且有效的方式来绘制和可视化全基因组信息,如GC含量、基因和重复序列密度、DNA甲基化分布、基因组共线性等。然而,大多数现有的软件程序和网络服务器仅适用于少数模式物种,如人类、小鼠和果蝇,或者应用场景有限。随着越来越多的非模式物种进行了染色体水平的组装测序,需要能够为广泛物种生成染色体模式图并能够可视化更多数据类型的工具,以帮助更好地理解基本的基因组特征。

结果

R包允许用户构建任何感兴趣物种的高质量染色体模式图。它可以在染色体模式图上绘制连续和离散的全基因组数据,并分别以热图和轨道标签的形式进行可视化。

结论

全基因组数据映射和比较的可视化使用户能够快速建立对染色体分布模式的清晰印象,从而成为任何从事组学研究的人员的有用工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25b9/7924719/021ca74e98ec/peerj-cs-06-251-g001.jpg

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