Chong Pan Pan, Selvaratnam Lakshmi, Abbas Azlina A, Kamarul Tunku
Tissue Engineering Group, National Orthopaedic Centre of Excellence for Research and Learning, Department of Orthopaedic Surgery, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Jeffrey Cheah School of Medicine and Health Sciences, Monash University Malaysia, Jalan Lagoon Selatan, 46150, Bandar Sunway, Selangor, Malaysia.
Open Life Sci. 2018 Aug 21;13:279-284. doi: 10.1515/biol-2018-0034. eCollection 2018 Jan.
Most studies highlight mesenchymal stem cells (MSCs) extracted primarily from bone marrow (BM), very few report the use of peripheral blood (PB), often due to the associated low seeding density and difficulties with extraction techniques. As ageing populations are becoming more predominant globally, together with escalating demands for MSC transplantation and tissue regeneration, obtaining quality MSCs suitable for induced differentiation and biological therapies becomes increasingly important. In this study, BM and PB were obtained from elderly patients and extracted MSCs grown to determine their successful isolation and expansion. Patients' socio-demographic background and other medical information were obtained from medical records. Successful and failed cultures were correlated with key demographic and medical parameters. A total of 112 samples (BM or PB) were used for this study. Of these, 50 samples (44.6%) were successfully cultured according to standardised criteria with no signs of contamination. Our comparative analyses demonstrated no statistical correlation between successful MSC cultures and any of the six demographic or medical parameters examined, including sample quantity, age, sex, race, habits and underlying comorbidities of sample donors. In conclusion, the present study demonstrates that typical demographics and comorbidities do not influence successful MSC isolation and expansion in culture.
大多数研究都强调主要从骨髓（BM）中提取间充质干细胞（MSC），很少有报告使用外周血（PB），这通常是由于外周血相关的低接种密度以及提取技术上的困难。随着全球老龄化人口日益占主导地位，加上对MSC移植和组织再生的需求不断增加，获取适合诱导分化和生物治疗的优质MSC变得越来越重要。在本研究中，从老年患者身上获取骨髓和外周血，并提取培养生长的MSC，以确定其成功分离和扩增情况。患者的社会人口背景和其他医疗信息从病历中获取。成功和失败的培养与关键人口统计学和医学参数相关。本研究共使用了112个样本（骨髓或外周血）。其中，50个样本（44.6%）根据标准化标准成功培养，没有污染迹象。我们的比较分析表明，成功的MSC培养与所检查的六个人口统计学或医学参数中的任何一个之间均无统计学相关性，这些参数包括样本量、年龄、性别、种族、习惯以及样本供体的潜在合并症。总之，本研究表明，典型的人口统计学特征和合并症不会影响培养中MSC的成功分离和扩增。
