Jiang Dongmei, Mo Guilin, Jiang Yilong, Kang Bo
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, People's Republic of China.
Open Life Sci. 2021 Jan 20;16(1):39-45. doi: 10.1515/biol-2021-0006. eCollection 2021.
Spermidine is important for the hypothalamic control of pituitary secretion of hormones involved in neuroendocrine functions in mammals. In this study, the effect of exogenous spermidine on the expression of genes and proteins related to polyamine metabolism and polyamine levels was examined. The results indicated that treatment with spermidine at 0.05 mg/g (BW) significantly increased the levels of 1 mRNA and protein expression and decreased putrescine content in mouse hypothalamus ( < 0.05). The administration with spermidine at 0.10 mg/g significantly increased the levels of 1, 2, and expression in mouse hypothalamus ( < 0.05). Treatment with spermidine at 0.05 mg/g significantly increased the levels of mRNA expression and reduced the level of mRNA expression in mouse hypothalamus ( < 0.05). Putrescine concentrations in the hypothalamus after the administration of spermidine at 0.10 and 0.15 mg/g were significantly higher than those in the control group ( < 0.05). The concentration of both spermidine and spermine in the hypothalamus after the administration of spermidine at 0.15 mg/g was decreased significantly ( < 0.05). In summary, our results indicate that exogenous spermidine affects polyamine homeostasis in the mouse hypothalamus by modulating the expression of genes and proteins related to polyamine metabolism.
亚精胺对于哺乳动物下丘脑控制参与神经内分泌功能的垂体激素分泌至关重要。在本研究中，检测了外源性亚精胺对与多胺代谢相关的基因和蛋白质表达以及多胺水平的影响。结果表明，以0.05 mg/g（体重）的剂量用亚精胺处理可显著提高小鼠下丘脑1的mRNA和蛋白质表达水平，并降低腐胺含量（<0.05）。以0.10 mg/g的剂量给予亚精胺可显著提高小鼠下丘脑1、2和的表达水平（<0.05）。以0.05 mg/g的剂量用亚精胺处理可显著提高小鼠下丘脑mRNA表达水平并降低mRNA表达水平（<0.05）。以0.10和0.15 mg/g的剂量给予亚精胺后，下丘脑腐胺浓度显著高于对照组（<0.05）。以0.15 mg/g的剂量给予亚精胺后，下丘脑亚精胺和精胺的浓度均显著降低（<0.05）。总之，我们的结果表明，外源性亚精胺通过调节与多胺代谢相关的基因和蛋白质表达来影响小鼠下丘脑的多胺稳态。
