Kang Bo, Jiang Dongmei, Ma Rong, He Hui, Yi Zhixin, Chen Ziyu
College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan Province, People's Republic of China.
Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan Province, People's Republic of China.
PLoS One. 2017 Mar 31;12(3):e0175016. doi: 10.1371/journal.pone.0175016. eCollection 2017.
An increasing number of studies suggest that ornithine decarboxylase antizyme 1 (OAZ1), which is regarded as a tumor suppressor gene, regulates follicular development, ovulation, and steroidogenesis. The granulosa cells in the ovary play a critical role in these ovarian functions. However, the action of OAZ1 mediating physiological functions of granulosa cells is obscure. OAZ1 knockdown in granulosa cells of geese was carried out in the current study. The effect of OAZ1 knockdown on polyamine metabolism, cell proliferation, apoptosis, and hormone receptor transcription of primary granulosa cells in geese was measured. The viability of granulosa cells transfected with the shRNA OAZ1 at 48 h was significantly higher than the control (p<0.05). The level of putrescine and spermidine in granulosa cells down-regulating OAZ1 was 7.04- and 2.11- fold higher compared with the control, respectively (p<0.05). The CCND1, SMAD1, and BCL-2 mRNA expression levels in granulosa cells down-regulating OAZ1 were each significantly higher than the control, respectively (p<0.05), whereas the PCNA and CASPASE 3 expression levels were significantly lower than the control (p<0.05). The estradiol concentration, ER and LHR mRNA expression levels were significantly lower in granulosa cells down-regulating OAZ1 compared with the control (p<0.05). Taken together, our results indicated that OAZ1 knockdown elevated the putrescine and spermidine contents and enhanced granulosa cell viability and inhibited ER and LHR transcriptions of granulosa cells in geese.
越来越多的研究表明，鸟氨酸脱羧酶抗酶1（OAZ1）被视为一种肿瘤抑制基因，可调节卵泡发育、排卵和类固醇生成。卵巢中的颗粒细胞在这些卵巢功能中起关键作用。然而，OAZ1介导颗粒细胞生理功能的作用尚不清楚。本研究对鹅颗粒细胞进行了OAZ1基因敲低。检测了OAZ1基因敲低对鹅原代颗粒细胞多胺代谢、细胞增殖、凋亡及激素受体转录的影响。48小时时，用shRNA OAZ1转染的颗粒细胞活力显著高于对照组（p<0.05）。下调OAZ1的颗粒细胞中腐胺和亚精胺水平分别比对照组高7.04倍和2.04倍（p<0.05）。下调OAZ1的颗粒细胞中CCND1、SMAD1和BCL-2 mRNA表达水平均显著高于对照组（p<0.05），而PCNA和CASPASE 3表达水平显著低于对照组（p<0.05）。下调OAZ1的颗粒细胞中雌二醇浓度、ER和LHR mRNA表达水平均显著低于对照组（p<0.05）。综上所述，我们的结果表明，OAZ1基因敲低提高了腐胺和亚精胺含量，增强了颗粒细胞活力，并抑制了鹅颗粒细胞的ER和LHR转录。
