Tykałowski Bartłomiej, Śmiałek Marcin, Kowalczyk Joanna, Dziewulska Daria, Stenzel Tomasz, Koncicki Andrzej
Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Warmia and Mazury, 10-719 Olsztyn, Poland.
J Vet Res. 2021 Jan 29;65(1):79-85. doi: 10.2478/jvetres-2021-0010. eCollection 2021 Mar.
Turkey histomonosis poses a serious threat to poultry production due to the ban on the use of effective drugs. The aim of the study was to evaluate the influence of a phytoncidal feed supplement on the course of histomonosis. The preparation was also analysed for immunomodulatory properties.
Clinical observations and production monitoring were conducted in a flock of turkeys with histomonosis from their 11 to 56 weeks of life which were treated with the adiCoxPF soluble supplement in a dose of 2.5 mL/L water. Later the preparation was used in a preventive dose (1 mL/L). The influence on the immune system was evaluated in broiler turkeys having been given adiCoxPF for 3 days in doses of 1 or 3 mL/L. The T and B lymphocyte percentages in turkey blood and spleen tissue were analysed with flow cytometry. ELISA was implemented to evaluate antibody titres after vaccination, and biochemical analyses were performed to evaluate the supplement's safety.
AdiCoxPF was found effective in therapy and prevention of histomonosis. Additionally, adiCoxPF stimulated both humoral and cell-mediated immune mechanisms, without impairing the functions of internal organs. The treated turkeys also yielded better production results (eggs/hen, fertility, and hatchability).
AdiCoxPF possesses immunomodulatory properties and it can be used successfully in the prevention and therapy of histomonosis in turkeys.
由于禁止使用有效药物，火鸡组织滴虫病对家禽生产构成严重威胁。本研究的目的是评估一种植物杀菌饲料添加剂对组织滴虫病病程的影响。还对该制剂的免疫调节特性进行了分析。
对一群患有组织滴虫病的火鸡进行了临床观察和生产监测，这些火鸡从11周龄到56周龄，用剂量为2.5 mL/L水的adiCoxPF可溶性添加剂进行治疗。之后，该制剂以预防剂量（1 mL/L）使用。对以1或3 mL/L剂量给予adiCoxPF 3天的肉鸡进行了免疫系统影响评估。用流式细胞术分析火鸡血和脾组织中的T和B淋巴细胞百分比。采用酶联免疫吸附测定法（ELISA）评估疫苗接种后的抗体滴度，并进行生化分析以评估该添加剂的安全性。
发现adiCoxPF在治疗和预防组织滴虫病方面有效。此外，adiCoxPF刺激了体液免疫和细胞介导的免疫机制，而不损害内脏器官的功能。经治疗的火鸡在生产性能方面也有更好的表现（产蛋量/母鸡、受精率和孵化率）。
adiCoxPF具有免疫调节特性，可成功用于预防和治疗火鸡组织滴虫病。
