Department of Ophthalmology, "Dr. Alexandru Popescu" Military Emergency Hospital Focșani, Focșani, Vrancea, Romania.
Department of Physiology, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
Rom J Ophthalmol. 2021 Jan-Mar;65(1):64-69. doi: 10.22336/rjo.2021.12.
The purpose of this experimental study was to evaluate the existence of adrenergic receptors in ketamine-induced corneal blood vessels in rat pups. The study of corneal neovascularization motricity was performed on 45-day-old Wistar rats in which, starting from the 15th day of life, corneal blood vessels were obtained by injecting intraperitoneal ketamine at a dose of 150 mg/ kg body weight, a total of 5 successive doses. The examination of the neovascularization was done with the help of a Nikon stereomicroscope connected to a video camera and a computer, the total magnification being 400X. The reactivity of the new corneal blood vessels to the administration in conjunctival instillations of a 1.5 mmol/L adrenaline solution was tested. The parameters followed were represented by variations in the caliber of corneal blood vessels. The data were analyzed using Microsoft Office Excel. Administration of distilled water did not produce statistically significant changes in corneal blood vessels, while adrenaline produced a statistically significant constriction of vascular diameter (p=0.01 at T9, p=0.004 at T10, p=0.019 at time T11 of examinations). The results showed that adrenaline produces vasoconstriction in the new corneal blood vessels, which allows us to assume that they contain α-adrenergic receptors. However, we cannot say that corneal pathological vessels do not contain β2-type adrenergic receptors, because the effect of adrenaline may be an algebraic sum between vasoconstriction produced by stimulating α-adrenergic receptors and vasodilation produced by stimulating β2-adrenergic receptors, but in which the vasodilating effect may be masked by the vasoconstrictor effect given by a higher density of α-adrenergic receptors. A= adrenaline, DNM = non-measurable diameter, NA= noradrenaline, Std.Er.= Standard error.
本实验研究的目的是评估氯胺酮诱导的幼鼠角膜血管中肾上腺素能受体的存在。对 45 日龄 Wistar 大鼠进行角膜新生血管运动性研究，从出生后第 15 天开始，通过腹腔注射氯胺酮，剂量为 150mg/kg 体重，共 5 次。新生血管的检查是在连接视频摄像机和计算机的 Nikon 立体显微镜的帮助下进行的，总放大倍数为 400X。通过在结膜滴注 1.5mmol/L 肾上腺素溶液来测试新角膜血管对给药的反应性。所跟踪的参数表现为角膜血管口径的变化。数据使用 Microsoft Office Excel 进行分析。蒸馏水给药不会导致角膜血管产生统计学上的显著变化，而肾上腺素会导致血管直径产生统计学上的显著收缩（T9 时 p=0.01，T10 时 p=0.004，T11 时 p=0.019）。结果表明，肾上腺素可引起新角膜血管收缩，这使我们假设它们含有α-肾上腺素能受体。然而，我们不能说角膜病理性血管不含有β2 型肾上腺素能受体，因为肾上腺素的作用可能是刺激α-肾上腺素能受体产生血管收缩和刺激β2-肾上腺素能受体产生血管舒张的代数和，但血管舒张作用可能被更高密度的α-肾上腺素能受体产生的血管收缩作用所掩盖。A=肾上腺素，DNM=不可测量的直径，NA=去甲肾上腺素，Std.Er.=标准误差。
