疟原虫感染细胞的分离与分级分离
The isolation and fractionation of malaria-infected cells.
作者信息
Kreier J P
出版信息
Bull World Health Organ. 1977;55(2-3):317-31.
Abstract
This paper is a critical review of procedures for the isolation of malarial parasites from host cells and their fractionation. The procedures are grouped according to the stage of parasite being isolated, and the procedures for isolation of the erythrocytic stages are further grouped by techniques used. Some types of procedure are described for isolation of all stages of the parasite, both those in the invertebrate and vertebrate hosts. The uses and limitations of the various procedures are described. It is concluded that all the procedures are useful for some purposes, but that from a morphological standpoint only natural release in culture and continuous flow oscillation provide large yields of intact erythrocytic parasites free of host cell membranes.
摘要
本文是对从宿主细胞中分离疟原虫及其分级分离程序的批判性综述。这些程序根据所分离疟原虫的阶段进行分组，而红细胞阶段的分离程序则进一步按所使用的技术进行分组。描述了一些用于分离寄生虫各个阶段（包括无脊椎动物和脊椎动物宿主中的阶段）的程序类型。阐述了各种程序的用途和局限性。得出的结论是，所有程序对于某些目的都是有用的，但从形态学角度来看，只有培养中的自然释放和连续流动振荡能提供大量不含宿主细胞膜的完整红细胞内疟原虫。
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