Isokawa K, Takagi M, Toda Y
Department of Anatomy, Nihon University School of Dentistry, Tokyo, Japan.
Anat Rec. 1988 Apr;220(4):369-75. doi: 10.1002/ar.1092200405.
The trout arterial wall contains numerous extracellular fibrils that are presumed to be elastic. However, the cytochemical properties of the arterial fibrils have not been studied. Thus, we have ultrastructurally and cytochemically examined these fibrils, utilizing routine uranyl acetate and lead (UA-Pb) double staining, the tannic acid (pH 7.0)-uranyl acetate (TA-UA) method as an electron-dense staining for elastin, and Thiéry's periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP) method to localize vicinal glycol-containing complex carbohydrates. The arterial fibrils, about 23 nm in thickness, were interwoven at random but frequently showed the circular alignment to the long axis of the aorta. Occasionally they appeared to coalesce side by side, and the coalesced portion tended to lose its affinity for UA-Pb stains. The TA-UA method stained the fibrils moderately to intensely and stained the coalesced parts of the fibrils more intensely. All of those TA-UA positive fibrils were completely removed after elastase en bloc digestion. The PA-TCH-SP method did not stain the arterial fibrils but stained another kind of much thinner interfibrillar filamentous structure. These results suggest that the fibrils in the wall of trout ventral aorta are elastin in nature and do not contain vicinal glycols, although the fibrils usually exist in a fibrillar form, which is unlike mammalian amorphous elastin.
鳟鱼的动脉壁含有许多被认为具有弹性的细胞外纤维。然而，动脉纤维的细胞化学特性尚未得到研究。因此，我们利用常规的醋酸铀和铅（UA-Pb）双重染色、作为弹性蛋白电子致密染色的单宁酸（pH 7.0）-醋酸铀（TA-UA）方法以及蒂埃里的高碘酸-硫代碳酰肼-银蛋白（PA-TCH-SP）方法对这些纤维进行了超微结构和细胞化学检查，以定位含邻位二醇的复合碳水化合物。动脉纤维的厚度约为23纳米，随机交织，但经常显示出与主动脉长轴呈圆形排列。偶尔它们似乎并排融合，融合部分往往会失去对UA-Pb染色的亲和力。TA-UA方法对纤维的染色程度为中度至强烈，对纤维的融合部分染色更强烈。所有这些TA-UA阳性纤维在弹性蛋白酶整体消化后完全消失。PA-TCH-SP方法未对动脉纤维染色，但对另一种更细的纤维间丝状结构进行了染色。这些结果表明，鳟鱼腹主动脉壁中的纤维本质上是弹性蛋白，不含邻位二醇，尽管这些纤维通常以纤维形式存在，这与哺乳动物的无定形弹性蛋白不同。
