Calvo J, Boya J, Borregon A, Garcia-Mauriño J E
Department of Histology, Faculty of Medicine, University Complutense, Madrid, Spain.
Anat Rec. 1988 Apr;220(4):424-8. doi: 10.1002/ar.1092200412.
Immunoperoxidase methods for the demonstration of three glial antigens, vimentin, glial fibrillary acidic protein, and S-100 protein, were applied to routine-fixed paraffin sections of rat pineal gland. A pre-embedding electron microscope immunoperoxidase method was also used to study the ultrastructural localization of S-100 protein in pineal cells. Light and electron microscopic results showed the presence of these antigenic glial markers in the second pineal cell type. The term glial cell is proposed for the second of parenchymatous cell in rat pineal gland.
采用免疫过氧化物酶法检测波形蛋白、胶质纤维酸性蛋白和S-100蛋白这三种神经胶质抗原，将其应用于大鼠松果体常规固定的石蜡切片。还采用包埋前电子显微镜免疫过氧化物酶法研究S-100蛋白在松果体细胞中的超微结构定位。光镜和电镜结果显示，这些抗原性神经胶质标志物存在于第二种松果体细胞类型中。本文提出将大鼠松果体实质细胞中的第二种细胞称为神经胶质细胞。
