López-Muñoz F, Boya J, Calvo J L, Marín F
Department of Histology, Faculty of Medicine, University Complutense, Madrid, Spain.
Histol Histopathol. 1992 Oct;7(4):643-6.
In the present work, the presence and distribution of astrocytes in the rat pineal stalk is investigated applying an immunohistochemical technique for the demonstration of glial fibrillary acidic protein (GFAP) on Epon-embedded semithin sections (0.5 micron thick). GFAP-immunoreactive cells are evenly and regularly distributed along the entire pineal stalk. The GFAP-immunoreactive cells display a stellate shape showing variable numbers of cell processes that are mainly oriented parallel to the longitudinal stalk axis. Astrocytic processes show a clear tendency to encircle the remaining elements of the pineal stalk; i.e., pinealocytes, nerve fibres and blood vessels. Furthermore, glial processes form a cover layer separating the stalk from surrounding anatomical structures.
在本研究中，应用免疫组织化学技术，在包埋于Epon的半薄切片（0.5微米厚）上检测胶质纤维酸性蛋白（GFAP），以研究大鼠松果体柄中星形胶质细胞的存在和分布情况。GFAP免疫反应阳性细胞沿整个松果体柄均匀且规则地分布。GFAP免疫反应阳性细胞呈星形，具有数量不等的细胞突起，这些突起主要沿柄的纵轴平行排列。星形胶质细胞的突起明显倾向于环绕松果体柄的其余成分，即松果体细胞、神经纤维和血管。此外，胶质突起形成一层覆盖物，将松果体柄与周围的解剖结构分隔开来。
