Sheridan P J, Fujimoto M
Department of Cellular and Structural Biology, University of Texas Health Science Center, San Antonio 78284.
Anat Rec. 1988 Apr;220(4):442-5. doi: 10.1002/ar.1092200415.
There exists a sexual dimorphism in the occurrence of meningiomas. Biochemical binding assays conducted on samples of meningiomas have indicated a high incidence of progesterone and androgen receptors in these tumors. However, similar studies have been very controversial as to the existence of estrogen receptors in these tumors. The present study was conducted to determine whether the normal leptomenix contains estrogen and androgen receptors in a primate model, namely the baboon. Three male and three female baboons were injected with either 3H-dihydrotestosterone (3H-DHT) or 3H-estradiol. One animal from each group received 3H-steroid + 100-fold unlabeled corresponding steroid to serve as control. One hour after injection of the 3H-steroids the animals were sacrificed. Their brains were removed and processed for autoradiography. Nuclear uptake and retention of 3H-DHT and/or one of its metabolites was found in 25-50% of the cells in pieces of the arachnoid adhering to the brain, cells of the glial membrane, cells in large fiber bundles, presumably oligodendroglia, and cells lining the Virchow-Robins spaces. No such localization was found with 3H-estradiol. This study provides the first anatomical evidence for the presence of androgen receptors in the normal leptomenix and glial cells of the baboon. These findings are discussed in relation to the possible clinical significance of the use of steroids to modulate the growth of meningiomas.
脑膜瘤的发生存在性别差异。对脑膜瘤样本进行的生化结合试验表明，这些肿瘤中孕酮和雄激素受体的发生率很高。然而，关于这些肿瘤中雌激素受体的存在，类似的研究一直存在很大争议。本研究旨在确定在灵长类动物模型（即狒狒）中，正常软脑膜是否含有雌激素和雄激素受体。给三只雄性和三只雌性狒狒注射3H-双氢睾酮（3H-DHT）或3H-雌二醇。每组中的一只动物接受3H-类固醇+100倍未标记的相应类固醇作为对照。注射3H-类固醇一小时后，处死动物。取出它们的大脑并进行放射自显影处理。在附着于大脑的蛛网膜碎片中的25%-50%的细胞、神经胶质膜细胞、大纤维束中的细胞（可能是少突胶质细胞）以及维氏-罗宾斯间隙内衬的细胞中发现了3H-DHT和/或其一种代谢物的核摄取和保留。用3H-雌二醇未发现此类定位。本研究为狒狒正常软脑膜和神经胶质细胞中存在雄激素受体提供了首个解剖学证据。结合使用类固醇调节脑膜瘤生长的可能临床意义对这些发现进行了讨论。
