Gasc J M, Stumpf W E
J Embryol Exp Morphol. 1981 Jun;63:207-23.
The determinant role ascribed to steroid hormones in sexual differentiation of the reproductive tract of the embryo implies the presence of target cells for sex steroids. An autoradiographic technique adapted for diffusible compounds was employed to characterize and localize cells which concentrate either [3H]oestradiol (E2) or [3H]dihydrotestosterone (DHT) in their nuclei. This paper describes the topographical distribution of cells containing receptor sites for oestrogen or androgen in various tissues of the reproductive tract of chicken embryos from day 6 to 15 of incubation. Receptor sites for oestradiol are present in the mesenchyme of the cloaca and in urodeum and vascular body. In the lower part of the Wolffian duct, only epithelial cells display nuclear labelling. In the Müllerian duct, nuclear receptor sites for [3H]oestradiol are observed not before day 15. Receptor sites for DHT are localized in the mesenchyme of the cloacal region from day 7 to 15. The Wolffian, but not the Müllerian duct contains receptor sites for DHT in the nuclei of epithelial and mesenchymal cells. Cross-competition experiments between [3H]E2 or [3H]DHT and unlabelled DHT or E2 respectively, show that 2 different types of receptor sites exist. The observations indicate: (a) complementary roles forffian, but not the Müllerian duct contains receptor sites for DHT in the nuclei of epithelial and mesenchymal cells. Cross-competition experiments between [3H]E2 or [3H]DHT and unlabelled DHT or E2 respectively, show that 2 different types of receptor sites exist. The observations indicate: (a) complementary roles forffian, but not the Müllerian duct contains receptor sites for DHT in the nuclei of epithelial and mesenchymal cells. Cross-competition experiments between [3H]E2 or [3H]DHT and unlabelled DHT or E2 respectively, show that 2 different types of receptor sites exist. The observations indicate: (a) complementary roles for oestrogenic and androgenic hormones in embryonic sexual differentiation; (b) precocity of receptors for sex hormones during embryonic development; (c) importance of mesenchyme in differentiation processes which are sex-steroid dependent.
甾体激素在胚胎生殖道性分化中被赋予的决定性作用意味着存在性甾体的靶细胞。采用一种适用于可扩散化合物的放射自显影技术来表征和定位其细胞核中浓缩[3H]雌二醇（E2）或[3H]双氢睾酮（DHT）的细胞。本文描述了孵化第6天至15天鸡胚生殖道各组织中含有雌激素或雄激素受体位点的细胞的拓扑分布。雌二醇的受体位点存在于泄殖腔间充质、尿殖窦和血管体中。在沃尔夫管下部，只有上皮细胞显示核标记。在缪勒管中，[3H]雌二醇的核受体位点直到第15天才被观察到。DHT的受体位点在第7天至15天定位于泄殖腔区域的间充质中。沃尔夫管而非缪勒管在上皮细胞和间充质细胞核中含有DHT受体位点。[3H]E2或[3H]DHT与未标记的DHT或E2之间的交叉竞争实验分别表明存在2种不同类型的受体位点。这些观察结果表明：（a）雌激素和雄激素在胚胎性分化中的互补作用；（b）胚胎发育过程中性激素受体的早熟；（c）间充质在依赖性甾体的分化过程中的重要性。
