School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong, 255049, China.
J Food Sci. 2021 May;86(5):1802-1818. doi: 10.1111/1750-3841.15666. Epub 2021 Apr 6.
In the present study, antioxidant activities and functional properties of cowhide collagen antioxidant peptides (CCAPs) with different molecular weight (MW) were investigated. The optimum preparation conditions of CCAPs were hydrolysis time of 1.53 hr, temperature of 54.9 °C, pH 7.38, and neutral enzyme to trypsin ratio of 0.048 g: 0.016 g according to single factor test and response surface methodology (RSM). Three fractions (CCAP-I, CCAP-II, and CCAP-III) were obtained by ultrafiltration and lyophilization. Antioxidant activities revealed that CCAP-III had high reducing power activity (0.323 ± 0.035) and scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals (64.30 ± 5.99%), 2,2-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radicals (75.25 ± 3.14%), and hydroxyl radicals (68.26 ± 6.74%) compared to the other fractions. In addition, LC-MS/MS analysis showed that Ala-Gly-Glu-Arg, Gly-Ile-Ala-Gly-Glu-Arg, Gly-Pro-Ala-Gly-Pro-Ala-Gly-Pro-Arg, Gly-Val-Val-Gly-Pro-Glu-Gly-Ala-Arg and Gly-Phe-Ser-Gly-Leu-Asp-Gly-Ala-Lys were the major peptides of CCAP-III. CCAP-III showed good hygroscopicity (HYG), water holding capacity (WHC), and oil holding capacity (OHC) when compared with CCAP-I and CCAP-II. However, CCAP-II has great emulsifying properties, and CCAP-I has excellent foaming properties. Therefore, CCAPs can be used as a promising source of functional peptides with antioxidant properties. PRACTICAL APPLICATION: This study demonstrated the peptides of cowhide collagen has superior antioxidant and functional properties. This study provided a scientific basis for the preparation of antioxidant peptides from cowhide collagen.
在本研究中，研究了不同分子量（MW）的牛皮胶原蛋白抗氧化肽（CCAPs）的抗氧化活性和功能特性。根据单因素试验和响应面法（RSM），采用水解时间 1.53 小时、温度 54.9°C、pH7.38、中性酶与胰蛋白酶的比例为 0.048g：0.016g 作为最佳制备条件。通过超滤和冷冻干燥得到三个级分（CCAP-I、CCAP-II 和 CCAP-III）。抗氧化活性表明，CCAP-III 具有较高的还原力活性（0.323±0.035）和清除 2,2-二苯基-1-苦基肼（DPPH）自由基（64.30±5.99%）、2,2-偶氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基（75.25±3.14%）和羟基自由基（68.26±6.74%）的能力均高于其他级分。此外，LC-MS/MS 分析表明，Ala-Gly-Glu-Arg、Gly-Ile-Ala-Gly-Glu-Arg、Gly-Pro-Ala-Gly-Pro-Ala-Gly-Pro-Arg、Gly-Val-Val-Gly-Pro-Glu-Gly-Ala-Arg 和 Gly-Phe-Ser-Gly-Leu-Asp-Gly-Ala-Lys 是 CCAP-III 的主要肽段。与 CCAP-I 和 CCAP-II 相比，CCAP-III 具有较好的吸湿性（HYG）、持水能力（WHC）和持油能力（OHC）。然而，CCAP-II 具有良好的乳化性能，CCAP-I 具有优异的发泡性能。因此，CCAPs 可以作为具有抗氧化性能的功能性肽的有前途的来源。实际应用：本研究表明牛皮胶原蛋白肽具有优异的抗氧化和功能特性。本研究为从牛皮胶原蛋白制备抗氧化肽提供了科学依据。
