College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Veterinary Research Institute, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, 010031, China.
Sci Rep. 2024 Oct 28;14(1):25687. doi: 10.1038/s41598-024-75960-7.
Horse bone is rich in collagen, with a composition similar to that of human collagen. Collagen peptides supply nutrients needed for human growth that act as antioxidants, lower blood pressure. This study explored the extraction of collagen and the preparation of collagen short peptides from Mongolian horse bones. Bones were collected from horses of varying ages, and the collagen content along with calcium salt distribution were observed through staining and imaging analyses. Next, the bones were processed into a powder and then subjected to ultra-high-pressure processing for degreasing. The degreasing conditions were optimised by single-factor and orthogonal tests. Following this, collagen was extracted using an acid-enzymatic method, and its structural characteristics and thermal stability were assessed. The collagen short peptides were extracted from the collagen samples, and the effects of the enzymatic hydrolysis time, temperature, pH, and enzyme amount on the extraction rate were evaluated. Finally, the resulting collagen peptides were analysed for antioxidant activity. In summary, this experiment optimised the extraction conditions for horse bone collagen, demonstrating that the ultra-high-pressure method minimally affects collagen structure, and the extraction rate was high. Hence our method has significant development potential.
马骨富含胶原蛋白，其组成与人体胶原蛋白相似。胶原蛋白肽提供人体生长所需的营养物质，具有抗氧化、降血压的作用。本研究探索了从蒙古马骨中提取胶原蛋白和制备胶原蛋白短肽的方法。从不同年龄的马身上采集骨头，通过染色和成像分析观察胶原蛋白含量和钙盐分布。然后，将骨头加工成粉末，再进行超高压处理以去除油脂。通过单因素和正交试验优化了脱脂条件。接下来，采用酸酶法提取胶原蛋白，并对其结构特征和热稳定性进行了评估。从胶原蛋白样品中提取胶原蛋白短肽，并评估酶解时间、温度、pH 值和酶用量对提取率的影响。最后，分析了所得胶原蛋白肽的抗氧化活性。总之，本实验优化了马骨胶原蛋白的提取条件，表明超高压法对胶原蛋白结构的影响较小，提取率高。因此，我们的方法具有很大的发展潜力。
