Cao Jie, Xiong Ning, Zhang Yu, Dai Yuwei, Wang Yuye, Lu Lingyu, Jiang Lin
School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510006, China.
School of Public Health, Southern Medical University, Guangzhou 510515, China.
Foods. 2022 Mar 18;11(6):859. doi: 10.3390/foods11060859.
In this research, the neutrase hydrolysis conditions of edible bird's nest (EBN) by-products were optimized by response surface methodology (RSM). Antioxidant peptides were then isolated from the EBN by-products by ultrafiltration and chromatography taking the DPPH radical scavenging ability as an indicator. The antioxidant activity of the purified peptides was estimated by radical scavenging ability and sodium nitroprusside (SNP)-induced damage model in PC12 cells. When the enzyme concentration was10 kU/g-hydrolysis temperature was 45 °C, and hydrolysis time was 10.30 h, the degree of hydrolysis (DH) of EBN by-product hydrolysate (EBNH) was the highest. The purified peptide exerted strong scavenging ability with EC values of 0.51, 1.31, and 0.65 mg/mL for DDPH, ABTS, and O radicals, respectively. In addition, the purified peptides could significantly reduce the SNP-induced oxidative damage of PC12 cells, and twelve peptides that were rich in leucine (Leu), valine (Val), and lysine (Lys) were identified by LC-MS/MS. These results suggested that EBN by-products have potential as new materials for natural antioxidant peptides.
本研究采用响应面法（RSM）优化了食用燕窝（EBN）副产物的中性蛋白酶水解条件。然后以DPPH自由基清除能力为指标，通过超滤和色谱法从EBN副产物中分离出抗氧化肽。通过自由基清除能力和硝普钠（SNP）诱导的PC12细胞损伤模型评估纯化肽的抗氧化活性。当酶浓度为10 kU/g、水解温度为45℃、水解时间为10.30 h时，EBN副产物水解物（EBNH）的水解度（DH）最高。纯化肽对DDPH、ABTS和O自由基的清除能力较强，EC值分别为0.51、1.31和0.65 mg/mL。此外，纯化肽可显著降低SNP诱导的PC12细胞氧化损伤，并通过LC-MS/MS鉴定出12种富含亮氨酸（Leu）、缬氨酸（Val）和赖氨酸（Lys）的肽。这些结果表明，EBN副产物有潜力作为天然抗氧化肽的新材料。
