Department of Biomedical Engineering, The University of Akron, Ohio, USA.
Department of Bioengineering, University of Texas at Arlington, Arlington, Texas, USA.
J Biomed Mater Res A. 2021 Oct;109(10):1922-1930. doi: 10.1002/jbm.a.37184. Epub 2021 Apr 6.
Cardiac extracellular matrix (cECM) derived hydrogel has been investigated to treat myocardial infarction through animal studies and clinical trials. The tissue harvesting site commonly selects porcine left ventricle (LV) because heart attack majorly takes place in LV. However, little is known about whether the region of cardiac tissue harvesting is critical for downstream applications. In this work, in vitro studies to compare cECM hydrogels derived from adult porcine whole heart (WH), LV, and right ventricle (RV) were performed. The cECM from WH has similar chemical composition compared with cECM from LV and RV. All three types of cECM hydrogels share many similarities in terms of their microstructure, gelation time, and mechanical properties. WH-derived cECM hydrogels have larger variations in storage modulus (G') and complex modulus (G*) compared with the other two types of cECM hydrogels. Both human cardiomyocytes and mesenchymal stem cells could maintain high cell viability on all hydrogels without significant difference. In terms of above results, the cECM hydrogels from WH, LV and RV exhibited similarity in material properties and cell response in vitro. Thus, future fabrication of cECM hydrogels from WH would increase the yield, which would decrease processing time and production cost.
心脏细胞外基质（cECM）衍生水凝胶已通过动物研究和临床试验被用于治疗心肌梗死。组织采集部位通常选择猪的左心室（LV），因为心脏病发作主要发生在 LV。然而，对于下游应用，心脏组织采集区域是否至关重要，目前知之甚少。在这项工作中，进行了体外研究以比较源自成年猪全心脏（WH）、LV 和右心室（RV）的 cECM 水凝胶。WH 的 cECM 与 LV 和 RV 的 cECM 具有相似的化学成分。所有三种类型的 cECM 水凝胶在微观结构、胶凝时间和机械性能方面具有许多相似之处。与其他两种类型的 cECM 水凝胶相比，WH 衍生的 cECM 水凝胶的储能模量（G'）和复模量（G*）变化更大。人心肌细胞和间充质干细胞在所有水凝胶上的细胞存活率均保持在较高水平，无显著差异。根据上述结果，WH、LV 和 RV 的 cECM 水凝胶在材料性能和细胞反应方面表现出相似性。因此，未来从 WH 制造 cECM 水凝胶将提高产量，从而缩短加工时间和降低生产成本。
