Department of Biochemistry, University of KwaZulu-Natal, Westville Campus, Durban 4000, South Africa; Department of Biochemistry, Veritas University, Bwari, Abuja, Nigeria.
Department of Biochemistry, University of KwaZulu-Natal, Westville Campus, Durban 4000, South Africa; Department of Pharmacology, University of the Free State, Bloemfontein 9300, South Africa.
Reprod Toxicol. 2021 Jun;102:24-34. doi: 10.1016/j.reprotox.2021.03.007. Epub 2021 Apr 3.
Testicular dysfunctions leading to male infertility has been reported in type 2 diabetes (T2D), with glucose dysmetabolism, cholinergic and purinergic dysfunction being major contributors. In the present study, the effect of vanillin on glucose metabolism, purinergic and cholinergic dysfunctions were investigated in testicular tissues of T2D rats. Male Sprague-Dawley rats were divided into 6 groups containing 5 rats each. T2D was induced in rats by administering 10 % fructose ad libitum for 14 days followed by a single intraperitoneal injection (40 mg/kg body weight) of streptozotocin. T2D rats were orally administered with vanillin at 150 and 300 mg/kg body weight (bw). Diabetic control (DC) consisted of untreated diabetic rats, while normal control (NC) consisted of normal rats and they were administered with distilled water only. Metformin was used as the standard antidiabetic drug. After 5 weeks treatment, the rats were sacrificed, and the testes were harvested. Induction of T2D led to significantly depleted testicular levels of glutathione, glycogen content, superoxide dismutase and catalase enzyme activities, with concomitantly elevated levels of nitric oxide, malondialdehyde, acetylcholinesterase, glucose-6-phosphatase, fructose-1,6-biphophastase, glycogen phosphorylase, amylase and lipase activities. These activities and levels were significantly reversed to near normal in rats treated with both doses of vanillin as compared with metformin. These results, when taken together, suggest the therapeutic effect of vanillin against hyperglycemia-mediated metabolic dysfunctions in testes of T2D rats. This is depicted by the ability of the phenolic to attenuate oxidative imbalance, purinergic and cholinergic dysfunctions, while suppressing glucose dysmetabolism.
已报道 2 型糖尿病（T2D）可导致睾丸功能障碍进而引起男性不育，葡萄糖代谢紊乱、胆碱能和嘌呤能功能障碍是主要原因。在本研究中，研究了香草醛对 T2D 大鼠睾丸组织中葡萄糖代谢、嘌呤能和胆碱能功能障碍的影响。雄性 Sprague-Dawley 大鼠分为 6 组，每组 5 只。14 天内自由给予 10%果糖诱导大鼠 T2D，随后单次腹腔注射链脲佐菌素（40mg/kg 体重）。T2D 大鼠分别口服给予香草醛 150 和 300mg/kg 体重（bw）。糖尿病对照组（DC）由未经治疗的糖尿病大鼠组成，正常对照组（NC）由正常大鼠组成，仅给予蒸馏水。二甲双胍用作标准抗糖尿病药物。治疗 5 周后，处死大鼠，采集睾丸。T2D 的诱导导致睾丸中谷胱甘肽、糖原含量、超氧化物歧化酶和过氧化氢酶活性显著降低，同时一氧化氮、丙二醛、乙酰胆碱酯酶、葡萄糖-6-磷酸酶、果糖-1,6-二磷酸酶、糖原磷酸化酶、淀粉酶和脂肪酶活性升高。与二甲双胍相比，香草醛两种剂量处理的大鼠上述活性和水平均显著恢复正常。这些结果表明，香草醛具有治疗 T2D 大鼠高血糖介导的睾丸代谢功能障碍的作用。这表现在酚类物质能够减轻氧化失衡、嘌呤能和胆碱能功能障碍，同时抑制葡萄糖代谢紊乱。
