Phytomedicine and Molecular Toxicology Research Laboratory, Department of Biochemistry, Federal University Oye-Ekiti, Oye-Ekiti, Ekiti State, Nigeria.
Institute of Drug Research and Development, SE Bogoro Center, Afe Babalola University, Ado-Ekiti, Nigeria.
Metab Brain Dis. 2024 Oct;39(7):1353-1362. doi: 10.1007/s11011-024-01386-9. Epub 2024 Aug 2.
The neuroprotective ability of alkaloid-rich leaf extract of Dalbergiella welwitschii in streptozotocin-induced type 2 diabetic rats were investigated in this study. Dalbergiella welwitshii leaf alkaloid-rich extract was obtained using standard procedure. Streptozotocin was injected into the experimental animals intraperitoneally at a dose of 45 mg/mg body weight. Prior to this, the animals were given 20% (w/v) fructose for one week. The animals were grouped into five (n = 8), comprising of normal control (NC), diabetic control (DC), diabetic rats treated with low (50 mg/mg body weight) and high (100 mg/kg body weight) doses of Dalbergiella welwitschii alkaloid-rich leaf extracts (i.e., DWL and DWH respectively) and 200 mg/kg body weight of metformin (MET). The animals were sacrificed on the 21st day, blood and brain tissue were harvested and used for the determination of neurotransmitters, cholinesterase, some ATP activities, oxidative stress biomarkers and histological examination. The results show that diabetic rats placed on DWL, DWH and MET significantly (p < 0.05) reduced cholinergic, elevated some ATPase activities and ameliorated oxidative stress biomarkers. These were supported by the histological examination by improving neuroprotective effects in diabetic rats administered DWL, DWH and MET. Hence, it can be presumed that DWL and DWH could be beneficial in treating diabetic neurodegenerative diseases.
本研究旨在探讨富含生物碱的 Dalbergiella welwitschii 叶提取物对链脲佐菌素诱导的 2 型糖尿病大鼠的神经保护作用。采用标准程序从 Dalbergiella welwitschii 叶中提取富含生物碱的提取物。将链脲佐菌素以 45mg/mg 体重的剂量腹腔注射到实验动物体内。在此之前，动物被给予 20%（w/v）果糖一周。将动物分为五组（n=8），包括正常对照组（NC）、糖尿病对照组（DC）、分别用低（50mg/mg 体重）和高（100mg/kg 体重）剂量的 Dalbergiella welwitschii 富含生物碱的叶提取物（即 DWL 和 DWH）以及 200mg/kg 体重的二甲双胍（MET）处理的糖尿病大鼠。在第 21 天处死动物，采集血液和脑组织，用于测定神经递质、胆碱酯酶、部分 ATP 活性、氧化应激生物标志物和组织学检查。结果表明，给予 DWL、DWH 和 MET 的糖尿病大鼠的胆碱能显著降低（p<0.05），某些 ATPase 活性升高，氧化应激生物标志物得到改善。这些结果得到了 DWL、DWH 和 MET 处理的糖尿病大鼠神经保护作用改善的组织学检查的支持。因此，可以推测 DWL 和 DWH 可能有益于治疗糖尿病神经退行性疾病。
