Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Ciudad de México, Mexico.
Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Ciudad de México, Mexico; Current address Environmental Health Science and Research Bureau, Health Canada, Ottawa, Canada.
Environ Toxicol Pharmacol. 2021 Aug;86:103654. doi: 10.1016/j.etap.2021.103654. Epub 2021 Apr 3.
The increasing use of metal oxide nanoparticles (MONPs) as TiO NPs or ZnO NPs has led to environmental release and human exposure. The respiratory system, effects on lamellar bodies and surfactant protein A (SP-A) of pneumocytes, can be importantly affected. Exposure of human alveolar epithelial cells (A549) induced differential responses; a higher persistence of TiO in cell surface and uptake (measured by Atomic Force Microscopy) and sustained inflammatory response (by means of TNF-α, IL-10, and IL-6 release) and ROS generation were observed, whereas ZnO showed a modest response and low numbers in cell surface. A reduction in SP-A levels at 24 h of exposure to TiO NPs (concentration-dependent) or ZnO NPs (the higher concentration) was also observed, reversed by blocking the inflammatory response (by the inhibition of IL-6). Loss of SP-A represents a relevant target of MONPs-induced inflammatory response that could contribute to cellular damage and loss of lung function.
金属氧化物纳米粒子（MONPs）的使用日益增多，如 TiO2 NPs 或 ZnO NPs，这导致了它们在环境中的释放和人类的暴露。呼吸系统、对板层小体和肺泡细胞表面蛋白 A（SP-A）的影响可能会受到重要影响。人肺泡上皮细胞（A549）的暴露会引起不同的反应；通过原子力显微镜（Atomic Force Microscopy）测量，TiO2 在细胞表面的滞留和摄取（measured by Atomic Force Microscopy）更高，以及持续的炎症反应（通过 TNF-α、IL-10 和 IL-6 的释放）和 ROS 生成也更高，而 ZnO 则表现出适度的反应和细胞表面数量较少。暴露于 TiO2 NPs（浓度依赖性）或 ZnO NPs（较高浓度）24 小时后，SP-A 水平下降，通过抑制炎症反应（抑制 IL-6）也可逆转。SP-A 的丧失代表了 MONPs 诱导的炎症反应的一个相关靶点，可能导致细胞损伤和肺功能丧失。
