Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, PR China; Structural Genomics Consortium, University of Toronto, Toronto, Ontario, M5G 1L7, Canada; Department of Physiology, University of Toronto, Toronto, Ontario, M5S 1A8, Canada.
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences, Central China Normal University, Wuhan, 430079, PR China.
Curr Opin Chem Biol. 2021 Aug;63:105-114. doi: 10.1016/j.cbpa.2021.02.018. Epub 2021 Apr 3.
The chromatin structure is dynamically regulated by many different modulators that post-translationally modify histones, replace canonical histones with histone variants, and unwind nucleosomal DNA, thereby modulating the accessibility of nucleosomal DNA and facilitating downstream DNA-templated nuclear processes. To understand how these modulators change the chromatin structure, it is essential to determine the 3D structures of chromatin modulators in complex with nucleosome. Here, we review the very recent progress in structural studies of some selected chromatin modulators in complex with nucleosome, including those of histone demethylases LSD1/2, some pioneer transcription factors, and the PWWP domain-containing protein LEDGF.
染色质结构受许多不同的调节剂动态调控，这些调节剂通过翻译后修饰组蛋白、用组蛋白变体替代经典组蛋白、解开核小体 DNA，从而调节核小体 DNA 的可及性，并促进下游基于 DNA 的核过程。为了了解这些调节剂如何改变染色质结构，确定染色质调节剂与核小体复合物的 3D 结构是至关重要的。在这里，我们综述了一些选定的与核小体复合物的染色质调节剂的结构研究的最新进展，包括组蛋白去甲基酶 LSD1/2、一些先驱转录因子和含有 PWWP 结构域的蛋白 LEDGF。
