Ekpo Daniel Emmanuel, Joshua Parker Elijah, Odiba Arome Solomon, Nwodo Okwesilieze Fred Chiletugo
Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Federal Republic of Nigeria.
Department of Molecular Genetics and Biotechnology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Federal Republic of Nigeria.
Drug Chem Toxicol. 2022 Sep;45(5):1934-1950. doi: 10.1080/01480545.2021.1892957. Epub 2021 Apr 7.
P. Beauv. (Icacinaceae) is a good source of natural antioxidants, having the potential to protect against oxidative stress-related diseases and complications. This study investigated the antioxidant, hepatoprotective and curative effects of flavonoid-rich fraction of leaves (LAFRF) against carbon tetrachloride-induced hepatotoxicity in Wistar rats. Phytochemical, nutrient content, and antioxidant activity of LAFRF were determined by standard methods. Fifty Wistar rats were randomized into 10 groups ( = 5). Groups 1 and 2 served as normal and CCl controls, respectively. Groups 3A-6A constituted the protective study while groups 3B-6B represented the curative study. The effects of LAFRF at 3, 10, and 30 mg/kg body weight (b.w.) on lipid peroxidation, antioxidant status, liver enzymes activities, and histology of CCl-intoxicated rats were assessed. LAFRF total flavonoids (281.05 ± 7.44 mg QE/g), indicated LD above 5000 mg/kg b.w., and scavenged ABTS* with an IC of 5.05 ± 0.00 µg/mL relative to butylated hydroxytoluene (4.16 ± 0.00 µg/mL), and a concentration-dependent increase in total antioxidant capacity. Carbon tetrachloride (1 mL/kg) triggered significant ( < 0.05) increases in malonedialdehyde concentration (2.67 ± 0.21 mg/mL), with a corresponding decline in antioxidant status, and increases in alkaline phosphatase, alanine and aspartate aminotransferase activities (68.00 ± 9.59 IU/L, 79.60 ± 5.03 IU/L and 81.80 ± 3.96 IU/L), respectively. LAFRF significantly ( < 0.05) lowered lipid peroxidation levels, liver enzyme activities, increased antioxidant status, and improved hepatic histo-architecture of pre- and post LAFRF-treated rats. This demonstrates its high antioxidative, hepatoprotective and curative effects, indicating its potential for future drug development.
波叶滨榕（隶属于茶茱萸科）是天然抗氧化剂的优质来源，具有预防与氧化应激相关疾病及并发症的潜力。本研究调查了富含黄酮的叶片提取物（LAFRF）对四氯化碳诱导的Wistar大鼠肝毒性的抗氧化、保肝及治疗作用。采用标准方法测定了LAFRF的植物化学成分、营养成分及抗氧化活性。将50只Wistar大鼠随机分为10组（每组n = 5）。第1组和第2组分别作为正常对照组和四氯化碳对照组。第3A - 6A组为预防性研究组，第3B - 6B组为治疗性研究组。评估了LAFRF在3、10和30 mg/kg体重（b.w.）剂量下对四氯化碳中毒大鼠脂质过氧化、抗氧化状态、肝酶活性及组织学的影响。LAFRF总黄酮含量为（281.05 ± 7.44 mg QE/g），其半数致死量高于5000 mg/kg b.w.，相对于丁基羟基甲苯（4.16 ± 0.00 μg/mL），其对ABTS*的半数抑制浓度（IC）为5.05 ± 0.00 μg/mL，且总抗氧化能力呈浓度依赖性增加。四氯化碳（1 mL/kg）使丙二醛浓度显著（P < 0.05）升高（2.67 ± 0.21 mg/mL），抗氧化状态相应下降，碱性磷酸酶、丙氨酸氨基转移酶和天冬氨酸氨基转移酶活性分别升高（68.00 ± 9.59 IU/L、79.60 ± 5.03 IU/L和81.80 ± 3.96 IU/L）。LAFRF显著（P < 0.05）降低了脂质过氧化水平和肝酶活性，提高了抗氧化状态，并改善了LAFRF处理前后大鼠的肝脏组织结构。这表明其具有高度的抗氧化、保肝及治疗作用，显示出其在未来药物开发中的潜力。
