在以fura - 2负载的紫贻贝前足丝牵缩肌分离平滑肌细胞中，卡巴胆碱和氯化钾诱导的细胞内游离钙浓度变化。

Carbachol and KCl-induced changes in intracellular free calcium concentration in isolated, fura-2 loaded smooth-muscle cells from the anterior byssus retractor muscle of Mytilus edulis.

作者信息

Ishii N, Simpson A W, Ashley C C

机构信息

University Laboratory of Physiology, Oxford, U.K.

出版信息

Biochem Biophys Res Commun. 1988 Jun 16;153(2):683-9. doi: 10.1016/s0006-291x(88)81149-5.

DOI:10.1016/s0006-291x(88)81149-5

PMID:3382397

Abstract

Intracellular free calcium concentration [( Ca2+]1) was measured in suspensions of fura-2 loaded smooth-muscle cells isolated from the anterior byssus retractor muscle of Mytilus edulis. Successive application of 5mM carbachol (CCh) and 100mM KCl to the cells transiently elevated [Ca2+]1 from the resting value of 124 +/- 4.5nM (mean +/- S.E., n = 14) to 295 +/- 15.3 and 383 +/- 20.5 nM, respectively. The response to CCh was concentration-dependent with an ED50 of 10(-5) M. Under the microscope, 67 +/- 3.0 and 83 +/- 1.3 % of fura-2 loaded cells contracted on the addition of 5mM CCh and 100mM KCl, respectively. In Ca2+ -free sea water, the CCh induced change in [Ca2+]1 was partially suppressed whereas that induced by KCl was completely abolished, suggesting an agonist-evoked release of stored Ca2+.

摘要

在从贻贝前足缩肌分离出的、用fura - 2负载的平滑肌细胞悬液中测量细胞内游离钙浓度[（Ca2 +]i）。向细胞中依次施加5mM卡巴胆碱（CCh）和100mM KCl，使[Ca2 +]i从124±4.5nM（平均值±标准误，n = 14）的静息值分别短暂升高至295±15.3和383±20.5 nM。对CCh的反应呈浓度依赖性，ED50为10（-5）M。在显微镜下，分别添加5mM CCh和100mM KCl时，67±3.0%和83±1.3%的用fura - 2负载的细胞发生收缩。在无钙海水中，CCh诱导的[Ca2 +]i变化被部分抑制，而KCl诱导的变化则完全消除，提示激动剂诱发储存钙的释放。