Golovin S Ia, Bondar' A A, Karginov V A, Morozov I V, Zelenin S M
Bioorg Khim. 1988 Feb;14(2):273-5.
Total RNA was extracted from Mustella vison pituitary gland, and cDNA for proopiomelanocortin mRNA was synthesised and cloned. A 600 b. p. insert encoding for total ACTH, beta LPH and 3'-nontranslated end of the mRNA was sequenced using the Maxam-Gilbert technique.
从水貂垂体中提取总RNA,合成并克隆促肾上腺皮质激素原信使核糖核酸的互补脱氧核糖核酸。使用马克萨姆-吉尔伯特技术对编码总促肾上腺皮质激素、β-促脂素和信使核糖核酸3'-非翻译末端的600个碱基对插入片段进行测序。