[水貂（鼬属水貂）垂体中阿片促黑素细胞皮质素原cDNA的合成、克隆及一级结构]
[Synthesis, cloning and primary structure of cDNA for proopiomelanocortin from the pituitary gland of the mink (Mustella vison)].
作者信息
Golovin S Ia, Bondar' A A, Karginov V A, Morozov I V, Zelenin S M
出版信息
Bioorg Khim. 1988 Feb;14(2):273-5.
PMID:3382437
Abstract
Total RNA was extracted from Mustella vison pituitary gland, and cDNA for proopiomelanocortin mRNA was synthesised and cloned. A 600 b. p. insert encoding for total ACTH, beta LPH and 3'-nontranslated end of the mRNA was sequenced using the Maxam-Gilbert technique.
摘要
从水貂垂体中提取总RNA，合成并克隆促肾上腺皮质激素原信使核糖核酸的互补脱氧核糖核酸。使用马克萨姆-吉尔伯特技术对编码总促肾上腺皮质激素、β-促脂素和信使核糖核酸3'-非翻译末端的600个碱基对插入片段进行测序。
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