枸杞多糖改善干燥综合征小鼠模型。
Lycium barbarum Polysaccharide Ameliorates Sjögren's Syndrome in a Murine Model.
机构信息
Department of Rheumatology and Immunology, Peking University People's Hospital, Beijing, China.
Clinical Research Institute and Department of Interventional Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, China.
出版信息
Mol Nutr Food Res. 2021 Jun;65(11):e2001118. doi: 10.1002/mnfr.202001118. Epub 2021 May 5.
SCOPE
This study aims to evaluate the therapeutic efficacy and mechanisms of Lycium barbarum polysaccharide (LBP) in primary Sjögren's syndrome (pSS).
METHODS AND RESULTS
Non-obese diabetic mice (the pSS model) are randomly divided into four groups: Low dose LBP (LBP.L, 5 mg kg d ), high dose LBP (10 mg kg d ), low dose interleukin (IL)-2 (25 000 IU/d), and control (saline water). Drugs were treated for 12 weeks. LBP.L significantly reduces the salivary gland inflammation compared with the control group (histological score p vs = 0.019; foci number: p vs = 0.038). LBP.L also remarkably reduces the effector follicular helper T (Tfh) cells and the CD4 IL-17A helper T (Th17) cells in both spleen and cervical lymph node (cLN) cells. Additionally, the ratios of regulatory T cell (Treg)/Tfh cells and Treg/Th17 cells are substantially increased in mice treated with LBP.L in both spleen and cLNs. LBP also inhibits Th17 and Tfh cells and markedly increases the Treg/Tfh ratio in human peripheral blood mononuclear cells.
CONCLUSION
LBP.L inhibits the progression of pSS in mice, associated with modulation of T cell differentiation.
范围
本研究旨在评估枸杞多糖（LBP）在原发性干燥综合征（pSS）中的治疗效果和作用机制。
方法和结果
非肥胖型糖尿病（NOD）小鼠（pSS 模型）随机分为四组：低剂量 LBP（LBP.L，5mg/kg/d）、高剂量 LBP（10mg/kg/d）、低剂量白细胞介素（IL）-2（25000IU/d）和对照组（生理盐水）。药物治疗 12 周。与对照组相比，LBP.L 显著降低了唾液腺炎症（组织学评分 p vs = 0.019；灶数：p vs = 0.038）。LBP.L 还显著减少了脾脏和颈部淋巴结（cLN）细胞中的效应滤泡辅助性 T（Tfh）细胞和 CD4 白细胞介素-17A 辅助性 T（Th17）细胞。此外，LBP.L 处理的小鼠脾脏和 cLN 中的调节性 T 细胞（Treg）/Tfh 细胞和 Treg/Th17 细胞的比例均显著增加。LBP 还抑制 Th17 和 Tfh 细胞，并显著增加人外周血单个核细胞中的 Treg/Tfh 比例。
结论
LBP.L 抑制了小鼠 pSS 的进展，与 T 细胞分化的调节有关。
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