Centre for Biosensors, Bioelectronics and Biodevices (C3Bio), Department of Electronic and Electrical Engineering, University of Bath, Claverton Down, Bath, BA2 7AY, United Kingdom.
Centre for Regenerative Medicine, Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, BA2 7AY, United Kingdom.
Adv Biol (Weinh). 2021 Jun;5(6):e2100330. doi: 10.1002/adbi.202100330. Epub 2021 Apr 7.
Here shows that electrical impedance spectroscopy can be used as a non-invasive and real time tool to probe cell adhesion and differentiation from midbrain floor plate progenitors into midbrain neurons on Au electrodes coated with human laminin. The electrical data and equivalent circuit modeling are consistent with standard microscopy analysis and reveal that within the first 6 hours progenitor cells sediment and attach to the electrode within 40 hours. Between 40 and 120 hours, midbrain progenitor cells differentiate into midbrain neurons, followed by an electrochemically stable maturation phase. The ability to sense and characterize non-invasively and in real time cell differentiation opens up unprecedented avenues for implantable therapies and differentiation strategies.
这里表明，电阻抗谱可以作为一种非侵入性和实时工具，用于探测细胞黏附和分化，从中脑基板祖细胞到涂有人层粘连蛋白的 Au 电极上的中脑细胞。电数据和等效电路模型与标准显微镜分析一致，并揭示了在前 6 小时内，祖细胞沉降并在 40 小时内附着在电极上。在 40 到 120 小时之间，中脑细胞分化为中脑细胞，然后进入电化学稳定的成熟阶段。这种能够非侵入性和实时地感应和描述细胞分化的能力，为可植入治疗和分化策略开辟了前所未有的途径。
