脂多糖刺激的 BV2 小胶质细胞在用高丽参根甲醇提取物处理后的抗神经炎症反应。

Anti-neuroinflammatory responses in lipopolysaccharide-stimulated BV2 microglial cells upon treatment with methanol extract of Panax ginseng root.

机构信息

Division of Applied Biomedical Sciences and Biotechnology, School of Health Sciences, International Medical University, Bukit Jalil, Kuala Lumpur 57000, Malaysia.

Department of Agrotechnology and Biosciences, Malaysian Nuclear Agency, Bangi, Kajang 43000, Selangor, Malaysia.

出版信息

J Tradit Chin Med. 2021 Apr;41(2):185-193.

PMID:33825397

Abstract

OBJECTIVE

To investigate the anti-neuroinflammatory properties of Panax ginseng (P. ginseng) root by measuring the levels of nitric oxide (NO), tumour necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-10 (IL-10) in lipopolysaccharide (LPS)-stimulated BV2 microglia cells.

METHODS

Maximal non-toxic dose (MNTD) of methanol extract of P. ginseng root culture on BV2 microglia cells was first determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, followed by treatment and LPS stimulation of cells, and the measurement of NO using Griess assay and TNF-α, IL-6, and IL-10 using ELISA assay.

RESULTS

The MNTD of P. ginseng root extract was determined to be (587 ± 57) µg/mL. Following that, NO and IL-6 levels were found to be insignificantly reduced by 6.88% and 0.14% respectively in stimulated cells upon treatment with MNTD. Treatment with MNTD yielded similar insignificant result, with only a reduction of 3.58% and 0.08% in NO and IL-6 levels respectively. However, TNF-α and IL-10 levels were significantly downregulated by 15.64% and 34.96% respectively upon treatment with P. ginseng root extract at MNTD.

CONCLUSION

Methanol extract of P. ginseng root culture did not show any significant anti-inflammatory effects on NO and IL-6 levels, but might potentially possess both anti-neuroinflammatory and pro-neuroinflammatory properties through the downregulation of TNF-α and IL-10 respectively.

摘要

目的

通过测量脂多糖（LPS）刺激的 BV2 小胶质细胞中一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-10（IL-10）的水平，研究人参（P. ginseng）根的抗神经炎症特性。

方法

首先通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐测定法测定人参根培养物甲醇提取物对 BV2 小胶质细胞的最大无毒剂量（MNTD），然后对细胞进行处理和 LPS 刺激，并使用 Griess 测定法测定 NO，使用 ELISA 测定法测定 TNF-α、IL-6 和 IL-10。

结果

确定人参根提取物的 MNTD 为（587±57）µg/mL。随后，在刺激细胞中，MNTD 处理后 NO 和 IL-6 水平分别降低了 6.88%和 0.14%，但无统计学意义。MNTD 处理也产生了类似的无显著差异的结果，NO 和 IL-6 水平分别降低了 3.58%和 0.08%。然而，在 MNTD 下用人参根提取物处理时，TNF-α和 IL-10 水平分别显著下调了 15.64%和 34.96%。