Kang Seok Yong, Jung Hyo Won, Lee Mi-Young, Lee Hye Won, Chae Seong Wook, Park Yong-Ki
Department of Herbology, College of Korean Medicine, Dongguk University, 707, Sekjang-Dong, Gyeongju, Gyeongsangbuk-Do, 780-714, Republic of Korea.
Department of Herbology, College of Korean Medicine, Dongguk University, 707, Sekjang-Dong, Gyeongju, Gyeongsangbuk-Do, 780-714, Republic of Korea; Korean Medicine R&D Center, Dongguk University, 707, Sekjang-Dong, Gyeongju, Gyeongsangbuk-Do, 780-714, Republic of Korea.
Chin J Nat Med. 2014 Aug;12(8):573-81. doi: 10.1016/S1875-5364(14)60088-1.
To investigate the anti-inflammatory activities of the semen extract of Cuscuta chinensis Lam. (Cuscutae Semen; CS) on the production of inflammatory mediators, nitric oxide (NO), prostaglandin 2 (PGE2), and proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated BV-2 microglia.
BV-2 cells were treated with CS extract for 30 min, and then stimulated with LPS or without for 24 h. The levels of NO, PGE2 and proinflammatory cytokines were measured by Griess assay and ELISA. The expression of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX)-2 mRNA and protein was determined by RT-PCR and Western blot, respectively. The phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK), and the nuclear expression of nuclear factor (NF)-κB p65 were investigated by Western blot analysis.
CS extract significantly decreased the production of NO and PGE2 by suppressing the expression of iNOS and COX-2 in activated microglia. CS extract decreased the production of TNF-α, IL-1β, and IL-6 by down-regulating their transcription levels. In addition, CS extract suppressed the phosphorylation of ERK1/2, JNK, and p38 MAPK, and the nuclear translocation of NF-κB p65 in activated microglia.
These results indicate that CS extract is capable of suppressing the inflammatory response by microglia activation, suggesting that CS extract has potential in the treatment of brain inflammation.
研究菟丝子提取物（菟丝子；CS）对脂多糖（LPS）刺激的BV-2小胶质细胞中炎症介质、一氧化氮（NO）、前列腺素E2（PGE2）和促炎细胞因子产生的抗炎活性。
用CS提取物处理BV-2细胞30分钟，然后用LPS刺激或不刺激24小时。通过Griess法和酶联免疫吸附测定（ELISA）测量NO、PGE2和促炎细胞因子的水平。分别通过逆转录-聚合酶链反应（RT-PCR）和蛋白质印迹法测定诱导型一氧化氮合酶（iNOS）、环氧化酶（COX）-2 mRNA和蛋白质的表达。通过蛋白质印迹分析研究细胞外信号调节激酶1/2（ERK1/2）、c-Jun氨基末端激酶（JNK）和p38丝裂原活化蛋白激酶（MAPK）的磷酸化以及核因子（NF）-κB p65的核表达。
CS提取物通过抑制活化小胶质细胞中iNOS和COX-2的表达，显著降低了NO和PGE2的产生。CS提取物通过下调肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）和白细胞介素-6（IL-6）的转录水平，降低了它们的产生。此外，CS提取物抑制了活化小胶质细胞中ERK1/2、JNK和p38 MAPK的磷酸化以及NF-κB p65的核转位。
这些结果表明，CS提取物能够通过抑制小胶质细胞活化来抑制炎症反应，提示CS提取物在治疗脑部炎症方面具有潜力。
