Reichling S, Patel H V, Freeman K B, Kates A L, Himms-Hagen J
Department of Biochemistry, McMaster University, Hamilton, Ont., Canada.
Biochem Cell Biol. 1988 Mar;66(3):193-8. doi: 10.1139/o88-026.
The level of mRNA for uncoupling protein was measured in brown adipose tissue of young (8-10 weeks) and old (11 months) lean and ob/ob mice using a cDNA clone constructed previously. The level of poly(A)+ RNA was also measured using an oligo(dT)18 probe. Mice were kept at 28 degrees C or exposed to 14 degrees C for 12 h. The level of mRNA for uncoupling protein was normal in brown adipose tissue of younger obese mice but reduced in brown adipose tissue of old obese mice. The cold-induced absolute increase in uncoupling protein mRNA was smaller in obese mice, regardless of age. It is concluded that the known attenuation of the acute thermogenic response of brown adipose tissue of the ob/ob mouse to cold is accompanied by a similar attenuation of the initiation of the trophic response. It is likely, however, that these defects are secondary to the chronic reduction in sympathetic nervous system activity in brown adipose tissue of the ob/ob mouse, which results in a functional atrophy of the tissue.
使用先前构建的cDNA克隆，测定了年轻（8 - 10周龄）和年老（11月龄）的瘦型及ob/ob小鼠棕色脂肪组织中解偶联蛋白的mRNA水平。还使用oligo(dT)18探针测定了poly(A)+ RNA水平。小鼠饲养在28℃或暴露于14℃ 12小时。年轻肥胖小鼠棕色脂肪组织中解偶联蛋白的mRNA水平正常，但年老肥胖小鼠棕色脂肪组织中的该水平降低。无论年龄如何，肥胖小鼠中由寒冷诱导的解偶联蛋白mRNA的绝对增加量较小。得出的结论是，已知ob/ob小鼠棕色脂肪组织对寒冷的急性产热反应减弱伴随着营养反应起始的类似减弱。然而，这些缺陷可能继发于ob/ob小鼠棕色脂肪组织中交感神经系统活性的慢性降低，这导致了该组织的功能性萎缩。
