Griffin Samantha L, Chekan Jonathan R, Lira Justin M, Robinson Andrew E, Yerkes Carla N, Siehl Daniel L, Wright Terry R, Nair Satish K, Cicchillo Robert M
Corteva Agriscience, 9330 Zionsville Road, Indianapolis, Indiana 46268, United States.
Department of Biochemistry, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.
J Agric Food Chem. 2021 May 5;69(17):5096-5104. doi: 10.1021/acs.jafc.1c00439. Epub 2021 Apr 7.
Natural and modified versions of the 5-enolpyruvylshikimate-3-phosphate synthase () gene have been used to confer tolerance to the broad-spectrum herbicide glyphosate in a variety of commercial crops. The most widely utilized trait was obtained from the strain CP4 and has been commercialized in several glyphosate-tolerant crops. The EPSPS gene products are enzymes that have been divided into three classes based on sequence similarity, sensitivity to glyphosate, and steady-state catalytic parameters. Herein, we describe the informatics-guided identification and biochemical and structural characterization of a novel EPSPS from (DGT-28 EPSPS). The data suggest DGT-28 EPSPS and other closely related homologues exemplify a distinct new class (Class IV) of EPSPS enzymes that display intrinsic tolerance to high concentrations of glyphosate ( ≥ 5000 μM). We further demonstrate that , when transformed into stable plants, provides robust (≥4× field rates) vegetative/reproductive herbicide tolerance and has utility in weed-control systems comparable to that of commercialized events.
5-烯醇丙酮酰莽草酸-3-磷酸合酶（EPSPS）基因的天然版本和修饰版本已被用于使多种商业作物对广谱除草剂草甘膦产生耐受性。应用最为广泛的性状来自菌株CP4，并且已在几种抗草甘膦作物中实现商业化。EPSPS基因产物是一类酶，根据序列相似性、对草甘膦的敏感性和稳态催化参数可分为三类。在此，我们描述了来自嗜热栖热菌（DGT-28 EPSPS）的一种新型EPSPS的信息学引导鉴定以及生化和结构表征。数据表明，DGT-28 EPSPS和其他密切相关的同源物代表了一类独特的新型EPSPS酶（IV类），它们对高浓度草甘膦（≥5000 μM）具有内在耐受性。我们进一步证明，当转入稳定的植物中时，DGT-28 EPSPS可提供强大的（≥4倍田间用量）营养/生殖期除草剂耐受性，并且在杂草控制系统中的效用与商业化事件相当。
