Alshami Alanoud, Al Attas Rabab, Azzam Ahmad, Mohammed Amani, Al-Quhaidan Norah
Division of Pediatric Nephrology and Kidney Transplant, Multiorgan Transplant Center, King Fahad Specialist Hospital-Dammam/Saudi Arabia, Dammam, Saudi Arabia.
Division of Immunology, Department of Pathology and Laboratory Medicine, King Fahad Specialist Hospital, Dammam, Saudi Arabia, Dammam, Saudi Arabia.
BMC Nephrol. 2021 Apr 7;22(1):123. doi: 10.1186/s12882-021-02325-x.
The seroprevalence of SARS-CoV-2 infection has been studied in immunocompetent children. However, data in the pediatric kidney transplant population (PKT) are lacking.
Using two commercial immunoassays that measured IgG antibodies against SARS-CoV-2 spike protein and IgG against the nucleocapsid (N) protein, we screened 72 PKT recipients who attended the outpatient clinic for routine blood work. The majority of patients with positive serology underwent an additional serology test at least once during subsequent clinical follow-up. Patients were confirmed to have SARS-CoV-2 infection if they had two positive tests.
Eight patients out of the 72 screened (11.1%) had positive results for SARS-CoV-2 IgG antibodies in both serological tests. Of those who tested positive, 4 had positive SARS-CoV-2 PCR results before screening. All patients were asymptomatic or had a history of mild symptoms. All tested patients had persistently positive antibodies at a median follow-up time of 75 days (IQR, 44.5, 86.5 days). One patient had a positive PCR test at 75 days and a positive serology test at 120 days post infection.
The seroprevalence of SARS-CoV-2 was relatively high (11.1%) in our population. Although all patients were asymptomatic or mildly symptomatic, they mounted a strong humoral immune response that persisted for a few months despite being on triple immunosuppressants. These findings have positive implications regarding vaccination efficacy in this group.
已对免疫功能正常的儿童进行了严重急性呼吸综合征冠状病毒2（SARS-CoV-2）感染的血清流行率研究。然而，儿科肾移植人群（PKT）的数据尚缺。
我们使用两种商业免疫测定法，分别检测针对SARS-CoV-2刺突蛋白的IgG抗体和针对核衣壳（N）蛋白的IgG抗体，对72名到门诊进行常规血液检查的PKT受者进行了筛查。大多数血清学检测呈阳性的患者在随后的临床随访中至少接受了一次额外的血清学检测。如果患者两次检测均为阳性，则确诊为SARS-CoV-2感染。
在筛查的72名患者中，有8名（11.1%）在两次血清学检测中SARS-CoV-2 IgG抗体均呈阳性。在检测呈阳性的患者中，4名在筛查前SARS-CoV-2 PCR检测结果为阳性。所有患者均无症状或有轻度症状史。所有接受检测的患者在中位随访时间75天（四分位间距，44.5，86.5天）时抗体持续呈阳性。1名患者在感染后75天PCR检测呈阳性，120天时血清学检测呈阳性。
在我们的研究人群中，SARS-CoV-2的血清流行率相对较高（11.1%）。尽管所有患者均无症状或症状轻微，但他们产生了强烈的体液免疫反应，尽管使用了三联免疫抑制剂，这种反应仍持续了几个月。这些发现对该组人群的疫苗接种效果具有积极意义。
