Midgley J M, Watson D G, Healey T, Noble M
Department of Pharmacy, University of Strathclyde, Glasgow, UK.
Biomed Environ Mass Spectrom. 1988 May 1;15(9):479-83. doi: 10.1002/bms.1200150904.
Prednisolone, dexamethasone and betamethasone were labelled with deuterium via a simple synthetic procedure and used as internal standards in the gas chromatographic/mass spectrometric analysis of the corresponding undeuterated compounds. The mass spectrometer was used in the negative chemical ionization mode, which gave fragmentation of the methoxime trimethylsilyl ether derivatives favourable for their quantification. The method was applicable to the quantification of synthetic corticosteroids contained in human aqueous humour in the 0.1-10-ng range.
通过一个简单的合成程序，将泼尼松龙、地塞米松和倍他米松用氘标记，并用作相应未氘代化合物气相色谱/质谱分析中的内标。质谱仪采用负化学电离模式，这种模式使得甲氧肟三甲基硅醚衍生物的碎片化有利于它们的定量分析。该方法适用于定量测定人房水中含量在0.1 - 10纳克范围内的合成皮质类固醇。
